GENERATION OF DENDRITIC CELLS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY VOLUNTEERS AND BREAST CANCER PATIENTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DELIREZH N.; MOAZENI S.M.; SHOKRI F.; SHOKRGOZAR M.A.; ATRI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2005 | Volume:10 | Issue:Part 1 Page(s): 1-11

Download Full-Text

Persian Verion

View:

1,214

Download:

Cites:

Information Journal Paper

Title

GENERATION OF DENDRITIC CELLS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY VOLUNTEERS AND BREAST CANCER PATIENTS

Author(s)

Keywords

DENDRITIC CELLSQ3

GM-CSFQ4

IL-4Q3

BREAST CANCERQ2

Abstract

Objective: DENDRITIC CELLS (DC) are potent inducers of anti-tumor immune responses. There is increasing interest to use them in various fields of research and clinical treatment. In the present study we generated DCs from peripheral blood monocytes of healthy donors and BREAST CANCER patients and investigated their characteristics for use in research and immunotherapy. Materials and Methods: Plastic adherent peripheral blood mononuclear cells were cultured for 5 days in the presence of granulocyte macrophage colony stimulating factor (GM - CSF) and interleukin - 4 (IL -4) in RPMI 1640 supplemented with 10% FBS or human AB serum. The cells were then cultured for more 2 days with tumor necrosis factor-α (TNF - α) as maturation factor. Morphological analysis of the cells was made by light microscopy, phenotyping carried out using now cytometry and their capacity to stimulate allogenic and autologous mixed leukocyte reaction (MLR) determined by [3H]- thymidine uptake test. Results: After 5 days of culturing in the presence of GM - CSF and IL - 4 adherent PBMCs (mainly monocytes) appeared as loosely adherent clamps or isolated noating cells with the typical dendritic morphology. Analysis of their surface markers showed that the large cells are homogeneous and expressed high levels of CD1a, CD11c and HLA - DR and low level of CD14. Addition of TNF - α as a maturation factor for 2 days caused increase of cell size, membrane processes as well as expression of CD83 and HLA-DR. From the functional point of view these cells were potent in stimulating allogenic and autologous MLR (Mean stimulation Index (SI) was 20 and 8 respectively). Conclusion: On the basis of these results large numbers of nomocyte-derived DENDRITIC CELLS can be generated in vitro and pulsed with tumor-associated antigens to use as natural adjuvant in tumor immunotherapy and research.

Cites

No record.

References

No record.

Cite

APA: Copy

DELIREZH, N., MOAZENI, S.M., SHOKRI, F., SHOKRGOZAR, M.A., & ATRI, M.. (2005). GENERATION OF DENDRITIC CELLS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY VOLUNTEERS AND BREAST CANCER PATIENTS. KOWSAR MEDICAL JOURNAL, 10(Part 1), 1-11. SID. https://sid.ir/paper/32824/en

Vancouver: Copy

DELIREZH N., MOAZENI S.M., SHOKRI F., SHOKRGOZAR M.A., ATRI M.. GENERATION OF DENDRITIC CELLS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY VOLUNTEERS AND BREAST CANCER PATIENTS. KOWSAR MEDICAL JOURNAL[Internet]. 2005;10(Part 1):1-11. Available from: https://sid.ir/paper/32824/en

IEEE: Copy

N. DELIREZH, S.M. MOAZENI, F. SHOKRI, M.A. SHOKRGOZAR, and M. ATRI, “GENERATION OF DENDRITIC CELLS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY VOLUNTEERS AND BREAST CANCER PATIENTS,” KOWSAR MEDICAL JOURNAL, vol. 10, no. Part 1, pp. 1–11, 2005, [Online]. Available: https://sid.ir/paper/32824/en

Related Journal Papers