EFFECT OF MONO-2-ETHYLHEXYL) PHTHALATE ON INVITRO MATURATION RESUMPTION OF MEIOSIS AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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IMANI H.; DALMAN A.; SEPEHRI H.; KAZEMI S.; REZAZADEH M.; HASSANI F.; SHAHVERDI A.

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Journal: KOWSAR MEDICAL JOURNAL Year:2005 | Volume:10 | Issue:Part 1 Page(s): 37-44

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Title

EFFECT OF MONO-2-ETHYLHEXYL) PHTHALATE ON INVITRO MATURATION RESUMPTION OF MEIOSIS AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES

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Keywords

IN VITRO MATURATION

MEHP (MONO-2-ETHYL HEXYL) PHTHALATE

IMMATURE OOCYTE

MOUSE

Abstract

Objective: In this study of IN VITRO MATURATION, resumption of meiosis and development of immature MOUSE oocytes with MEHP were evaluated. Material and Methods: IMMATURE OOCYTEs recovered from NMRI MOUSE strain (4-6 weeks) in 4 different experimental groups and control groups. Control group: 198 IMMATURE OOCYTEs matured in MEM-α medium contain FCS5%, rFSH 100 mIU/ml hCG7.5 IU/ml sham's group: 203 IMMATURE OOCYTEs were matured in MEM-a medium containing FCS 5%, hCG 7.5 IU/ml, rFSH 100mIU/ml and DMSO 100µm. Group 1: 201 IMMATURE OOCYTEs were matured in MEM- α medium containing FCS5%, hCG 7.5 IU/ml rFSH 100mIU/ml and 50 MEHP5. Group 2: 209 IMMATURE OOCYTEs were matured in MEM- α medium containing FCS5%, hCG 7.5 IU, rFSH 100mIU/ml and 100 µm MEHP. Group 3: 203 IMMATURE OOCYTEs were matured in MEM- α medium containing FCS5%, hCG 7.5 IU/ml rFSH 100mIU/ml and 200µm MEHP. Group 4: 204 IMMATURE OOCYTEs were matured in MEM- α medium containing FCS5% , hCG 7.5 IU/ml rFSH 100mIU/ml and 400 µm MEHP. Fertilization and embryo development were done in T6 medium. Results: The resumption of meiosis in experimental groups was 75%, 63%, 60%, 56% spectively. The methaphase II in experimental groups was 53%, 49%, 42%, 37%. Maturation was significant compared with a control group (86%, 90% resumption of meiosis) and 49%, 75%. Embryo formation in the experimental groups were 60%, 57%, 44%, 28% decreased in compared with control group (74%, 61%). Conclusion: The results of this study showed that MEHP had negative effect on resumption of meiosis and IN VITRO MATURATION and development of immature MOUSE oocytes.

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APA: Copy

IMANI, H., DALMAN, A., SEPEHRI, H., KAZEMI, S., REZAZADEH, M., HASSANI, F., & SHAHVERDI, A.. (2005). EFFECT OF MONO-2-ETHYLHEXYL) PHTHALATE ON INVITRO MATURATION RESUMPTION OF MEIOSIS AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES. KOWSAR MEDICAL JOURNAL, 10(Part 1), 37-44. SID. https://sid.ir/paper/537892/en

Vancouver: Copy

IMANI H., DALMAN A., SEPEHRI H., KAZEMI S., REZAZADEH M., HASSANI F., SHAHVERDI A.. EFFECT OF MONO-2-ETHYLHEXYL) PHTHALATE ON INVITRO MATURATION RESUMPTION OF MEIOSIS AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES. KOWSAR MEDICAL JOURNAL[Internet]. 2005;10(Part 1):37-44. Available from: https://sid.ir/paper/537892/en

IEEE: Copy

H. IMANI, A. DALMAN, H. SEPEHRI, S. KAZEMI, M. REZAZADEH, F. HASSANI, and A. SHAHVERDI, “EFFECT OF MONO-2-ETHYLHEXYL) PHTHALATE ON INVITRO MATURATION RESUMPTION OF MEIOSIS AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES,” KOWSAR MEDICAL JOURNAL, vol. 10, no. Part 1, pp. 37–44, 2005, [Online]. Available: https://sid.ir/paper/537892/en

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