Journal of Shahid Sadoughi University of Medical Sciences
Year:2019 | Volume:27 | Issue:6 |Papers Count:9

Introduction: Schizophrenia is a mental disorder affecting 1% of the world's population. Two of genes have been recognized to be involved in development of this disease: DRD2 and GSTM1. Methods: This case-control study included 100 patients suffering from schizophrenia who referred to Yazd Neuropsychiatry Hospital. Also, 100 healthy patients without schizophrenia were selected as the control group. After DNA extraction, it was genotyped 100 schizophrenic and 100 healthy controls by use of restricted fragment of length polymorphism (RFLP) for Ser311Cys polymorphism and multiplex PCR for GSTM1. After performing relevant experiments and gaining some results, statistical analysis was performed using SPSS software16. In this study, Chi-square and logistic regression tests were used to investigate the relation between genotype of polymorphism and schizophrenia. Results: Data analysis showed that frequency distribution of Ser311Cys polymorphism genotypes between the patients and healthy participants was not significant (P: 0. 121). Also, for GSTM1, there was no association between the polymorphism and schizophrenia. In general, the frequency distribution of the deleting gene between the patients and the control group was not significant (P= 0. 089). And this polymorphism was significantly associated with symptoms (P = 0. 012). Conclusion: The results of this study show that Ser311Cys polymorphism and GSTM1 polymorphism is not common among the studied patients, therefore it indicates its non-effectiveness in the study population. However, because the study population is not representative of the entire Iranian population, further studies with larger population are needed.

Introduction: The purpose of the present study was to investigate the relationship between personality traits and psychological well-being in people with irritable bowel syndrome with mediating role of emotional regulation. Methods: Research method according to the applied purpose, and the data collection method was a descriptive correlation and a quantitative data type. The statistical population of this study was all the patients with irritable bowel syndrome referring to Danesh Gastrointestinal Clinic in Tehran in 1396. The sample size was 390 participants selected by calculate sample size in structural equations and using a sample purposive sampling method. In order to measure the variables, personality traits Neo Costa & McGary (1992), Psychological wellbeing Ryff (1989), and of emotional regulation Gross and John (2003) questionnaires were used. Evaluation of the proposed model was done using structural equation modeling and SPSS Inc., Chicago, IL; Version 18 as well as Amos. The reliability, convergent validity and divergent validity of the questionnaire, were investigated. Moreover, path coefficients and software significance coefficients were used to examine the research hypotheses. Results: The results showed that the degree of appropriateness of the proposed model was appropriate to the components of the research; personality traits and psychological well-being were found to be correlated with the role of mediator of emotional regulation in the patients with irritable bowel syndrome (p <0. 0001). Conclusion: This research confirms the relationship between personality traits and psychological well-being with the mediating role of emotional regulation in people with irritable bowel syndrome. Because of Numerous scientific evidence in the ontology of this syndrome, further study on emotional skills and related problems in people with irritable bowel syndrome are required.

Introdution: Newborn individualized developmental care and assessment program (NIDCAP) is emphasized. This study aimed to determining the barriers to implementation of NIDCAP from nurses' perspectives in neonatal intensive care units in Shahid Sadoughi Hospital in Yazd. Methods: This descriptive study was conducted using a census sampling method. The samples were ٥ ٤ nurses working in the intensive care unit of Shahid Sadoughi and Afshar Hospitals in Yazd. Data were gathered using two questionnaires, ( demographic and barrier assessment questionnaires). Data analysis was performed in SPSS version 24 using descriptive statistics, Chi-square, independent t-test and fisher test. Results: Results showed that in terms of mean and standard deviation, in the area barriers of sleep care, lack of skill (3. 08± 1. 28 ), In the area of pain and stress assessment management, lack of skill (2. 86. ± 1. 31), in the area of activities of daily living and family-centered care, lack of effective communication with parents respectively (2. 83 ± 1. 39) and ( 2. 86± 1. 32), in the area of provision of healing environment, lack of standard light and sound in unit (2. 76 ± 1. 81) and in the area of organizational barriers, the disproportionate number of nurses to the patients (3. 47± 0. 84) were identified as the most important barriers Conclusion: Based on the findings, lack of skill, lack of effective communication with parents, lack of standard environment and disproportionate number of nurses and patients are the most important barriers to the implementation of NIDCAP in NICUs that managers need to plan appropriate design for addressing them.

Introdution: Wound healing is a complex and dynamic process, which requires to be prepared a suitable environment to promote healing process. Wound dressing is one of the most important external factors affecting the wound healing process. With the advancement in technology, more than 1000 types of modern wound dressings have been developed. Wound dressings are either based on natural materials or on the basis of synthetic and chemical materials. In addition to the protective and covering role, which modern wound dressings play, they have a chemical and physical interaction with wound bed addition. They carry antibiotics, drugs and nanoparticles and play an important role in maintaining moisture or hydration the wound; all of these factors can facilitate the wound healing process. In this study, the wound healing process and the properties of wound dressings were presented first and then the types of wound dressings and their applications were investigated. This study, with comprehensive information on the types of modern wound dressings and their advantages and drawbacks, will help a physician in the proper selection of an appropriate wound dressing.

Introduction: Type 2 diabetes has many complications, including cardiovascular disease, which is associated with an increase in inflammatory biomarkers. Research has shown that physical activity can reduce inflammatory factors and improve cardiovascular disease. The purpose of this study was to investigate the effect of resistance exercise on some of the inflammatory and anti-inflammatory factors in the heart tissue of type 2 diabetic rats. Methods: In this study, 18 male rats were selected. Rats were diabetic with nicotinamide and streptozotocin (stz) and then randomly divided into 2 groups of control (n=9) and training (n=9). The training group performed a resistance training program for 8 weeks, 5 days a week. Measurement of TNF-α and IL10 expression in the heart tissue was determined using the one step SYBR TAKARA single-step kit. To compare the difference between the mean of variables between exercise and control groups, independent t-test and t-test were used also to determine the effect of diabetes induction on variables. To determine the relationship between variables, Pearson correlation coefficient and separation correlation coefficient were used. Data were analyzed by SPSS software version 22. Results: The results showed that the insulin resistance index decreased significantly in the training group compared to the control group. On the other hand, the expression of TNF-α gene decreased and IL10 showed a significant increase (α ≤ 0. 05). Conclusion: The results indicate that resistance training may be effective in modulating the inflammatory factors of TNF-α and IL10 in the heart tissue of type 2 diabetic rats.

Older adults demonstrate increased amounts of postural sway, which may ultimately lead to falls. The purpose of this systematic review was to investigate the effect of different foot orthoses on lower limb biomechanical variables, lower limb muscular activity, and balance in elderly people. Examining texts based on the search on the Magiran, Google Schoolar, Pubmed, Scopus, and SIVILICA sites were done by using following keywords: foot orthoses, footwear, aging, aged, elderly, Kinematic, walking, loading rate, Kinetic Walking, in combination in four part from 2005 to 2018. In the initial searches, 72 papers were obtained, eight articles based on the inclusion and exclusion criteria were selected. This systematic review demonstrated that the use of textures and modeling orthoses strengthens sensory receptors and improves postures, as well as improves the biomechanical parameters such as evertor and invertor moments and ground reaction forces in some cases.

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK، FAS، BAX، TNF-a، BCL-و 2 Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells. Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7 cells transfected with the pcDNA3. 1(+)-seb (recombinant) and pcDNA3. 1(+) (non-recombinant) plasmids and were selected by culturing in a selective medium of RPMI-1640 containing 600 μ g / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used. Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032). Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.

Introduction: Angiogenesis is the formation of new vessels from early vessels that has effected in pathologic condition such as growing tumor, metastases, arthritis rheumatoid, diabetes and physiologic condition such as developmental and growing of organ regeneration, wound healing, and reproduction. Otostegia presica is a member of Laminacea family, has antioxidant and anti-apoptosis properties. The aim of this study was to investigate the effects of hidroalkoholic extract of Otostegia presica on VEGFR expression and angiogenesis in chick chorioallantoic membrane. Methods: In this research, 60 fertilized Ross eggs were used and randomly were divided into five groups, included: control, sham-exposed, and three experimental groups treated with hidroalkoholic extract of Otostegia presica doses 100, 200, 400 mg/kg. On 2nd day of incubation, a window was opened for eggs under sterile condition. On 8th day, a gelatin sponge with 1×4×4 diameter was placed on chorioalantoic membrane (CAM) and extract fractions with dose of 100, 200, 400 mg/kg were added. On 12th day, length and weight of embryos were measured and CAM photos were captured. Then number and length of vessels in special area on CAM were measured with Image J. software. Sampling was performed for assessment of VEGFR expression. Data were analyzed by t-test and ANOVA and p<0. 05 was considered significant. Results: Comparison between mean of length and weight of embryos and number and length of vessels in controls and sham-exposed did not show significant difference (P>0. 05). Mean of the number of vessels showed significant increase in all experimental groups (P<0. 05). VEGFR expression has increased in experimental groups rather than sham-exposed group. Conclusion: The results showed that hidroalkoholic extract of Otostegia presica has have angiogenic effects and increased VEGFR expression in all experimental groups.