Change in the Expression of BAK 4FAS 4BAX 4TNF-A 4BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Afzali Sara; DOOSTI ABBAS; HEIDARI MANSOUR; Babaei Nahid; KESHAVARZ PARVANEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2019 | Volume:27 | Issue:6 Page(s): 1677-1685

Download Full-Text

Persian Verion

View:

528

Download:

Cites:

Information Journal Paper

Title

Change in the Expression of BAK 4FAS 4BAX 4TNF-A 4BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B

Author(s)

Keywords

Breast cancerQ2

Enterotoxin BQ1

ApoptosisQ1

MCF-7Q2

BAKQ1

FASQ1

BAXQ1

TNF-aQ1

BCL-2Q2

SurvivinQ2

Abstract

Introduction: Breast cancer is one of the most prevalent malignancies among women. Patients whose suffering from this condition, as a result of the use of conventional therapies often have a poor response to treatment and the relapse among them is frequent. In this study, the effects of staphylococcal enterotoxin type B on BAK, FAS, BAX, TNF-a, BCL-و 2 Survivin genes expression in human breast adenocarcinoma cells (MCF-7) was examined. Staphylococcal Enterotoxin B is a powerful member of the Staphylococcus aureus toxins family, which is known as an anticancer agent with potential for killing cancer cells. Methods: The experimental study was carried out at the Biotechnology Research Center of Islamic Azad University, Shahrekord Branch. By using Lipofectamine 2000 reagent, MCF-7 cells transfected with the pcDNA3. 1(+)-seb (recombinant) and pcDNA3. 1(+) (non-recombinant) plasmids and were selected by culturing in a selective medium of RPMI-1640 containing 600 μ g / mL antibiotic G418. Then, the expression of BAK, FAS, BAX, TNF-a, BCL-2, and Survivin genes in transfected cells were analyzed by real time PCR. Student's t-test, SPSS Inc., Chicago, IL; Version 16 and also Excel program for statistical analysis were used. Results: The results of this study indicated that staphylococcal enterotoxin type B (SEB) remarkably changes the expression of apoptotic related genes in MCF-7 cell line. It was observed a significant increase in the expression of BAK, FAS, BAX, and TNF-a genes, the expression of BCL-2 and Survivin genes significantly decreased compared to the control group (P=0/032). Conclusion: Staphylococcal enterotoxin type B has an inhibitory effect on the growth, proliferation and invasion of breast adenocarcinoma cells through altering the expression of the genes involved in the Apoptosis process. Therefore, it seems that there is a good research field for the use of this toxin in the control and treatment of human breast adenocarcinoma.

Cites

No record.

References

Cite

APA: Copy

Afzali, Sara, DOOSTI, ABBAS, HEIDARI, MANSOUR, Babaei, Nahid, & KESHAVARZ, PARVANEH. (2019). Change in the Expression of BAK 4FAS 4BAX 4TNF-A 4BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 27(6 ), 1677-1685. SID. https://sid.ir/paper/35978/en

Vancouver: Copy

Afzali Sara, DOOSTI ABBAS, HEIDARI MANSOUR, Babaei Nahid, KESHAVARZ PARVANEH. Change in the Expression of BAK 4FAS 4BAX 4TNF-A 4BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;27(6 ):1677-1685. Available from: https://sid.ir/paper/35978/en

IEEE: Copy

Sara Afzali, ABBAS DOOSTI, MANSOUR HEIDARI, Nahid Babaei, and PARVANEH KESHAVARZ, “Change in the Expression of BAK 4FAS 4BAX 4TNF-A 4BCL-2 and Survivin Apoptosis Genes of the Human Breast Adenocarcinoma Cells (MCF-7) by Staphylococcal Enterotoxin B,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 6 , pp. 1677–1685, 2019, [Online]. Available: https://sid.ir/paper/35978/en

Related Journal Papers