The purpose of this study was to compare the bone mineral density (BMD) in dominant and non-dominant leg between professional soccer players and non-athlete students. Fifteen professional soccer players (mean ± SD; age: 23.26±0.29 years, height: 174±1.07 cm, weight: 70.33±1.8 kg) and fourteen healthy non-athlete students (mean ± SD; age: 22 years, height: 173±1.2 cm, weight: 61.64±2.4 kg) participated in this study. All soccer players and control group were free of any illness such as diabetes, hyperthyroidism, hyperparathyroidism, cardiovascular disease and were not taking any medication. The activity levels and dietary habits of all subjects were noted. Bone mineral density (BMD) was measured by Dual Energy X-Ray Absorptiometry (DEXA) at the femoral neck, femoral trochanter of dominant and non-dominant legs. The participants did not report any anti-seizure drugs, alcohol and cortoon consumption, neither smoking cigarette. Paired sample ttest, independent t-test were used to analyses the data. The results of this study showed that non-dominant leg of soccer players had significantly higher BMD than their dominant leg (t= 2.92, P= 0.01), (1335 versus 1288 mg/cm²) no significant difference was observed between dominant and non-dominant leg of control group (t= 0.05, P= 0.95), (941.1 versus 941.5 mg/cm²). The level of BMD of both legs of soccer players were significantly higher than control group (t=8.03, t= 8.58, P= 0.000), (1335 and 1288 mg/cm² versus 941.5 and 941.5 mg/cm²). Because of more frequent engagement of non-dominant leg in take off, landing and stance in shooting, bone mineral density is higher than the other leg. Furthermore it seems that soccer play lead to increase of BMD in nondominant and dominant leg in soccer players, that this point cause useful in prevention of osteoporosis.