The purpose of this study was to investigate the effect of isotonic strength training on Electronorography (ENG) parameters in athletes. Thirty male college athletes (aged=22.33±1.26 years, height=175.80±6.31 cm, and weight=74.03±5.41 kg ) without history injuries of knee, ankle joint, gastrocnemius (GAS) muscle and other tendons and muscles involved in plantar flexion movement participated in this study. The subjects were randomly assigned to equal two groups: experimental (strength weight training) and control. Then the ENG parameters (Nerve Conduction Velocity (NCV), amplitude, latency time of M wave) and Maximal Voluntary Contraction (MVC) from the dominant leg of the subject were measured in pre-test. The experimental group performed calf raise movement 3 sessions per week for 8 weeks. During this period, the control group did not perform any specific type of regular exercise. At the end of this training period all parameters were measured again. t-student test was used for statistical analysis in significant level of p £0.05. The results indicated a significant increase in amplitude of M wave, MVC, NCV and a significant decrease in latency time of M wave in training groups but no significant change in these factors in control group. It was concluded that neural adaptation include nerve conduction velocity, increase of motor unit recruitment, firing rate and firing duration does occur in trained athletes when a new training stimulus is implemented.