Gene, Cell and Tissue
Year:2018 | Volume:5 | Issue:2|Papers Count:7

Background: Paratuberculosis, or Johne’ s disease, is caused by Mycobacterium avium subsp paratuberculosis (MAP) and affects the domestic and wild ruminants worldwide. Fecal culture as well as serological and molecular methods have been used for detection of MAP. Objectives: The aim of this study was to compare bovine fecal and buffy coat samples for diagnosis of Johne’ s disease based on PCR and to find out which one was more suitable for detection of MAP. Methods: Feces and blood samples were collected from 100 suspected cows with clinical signs of Johne’ s disease. DNA extraction was performed from fecal and blood samples by CTAB and proteinase K method. Polymerase chain reaction (PCR) was used to detect the presence of MAP. Results: In this study, 4 cases were positive in buffy coat PCR test and 9 cases were positive in feces PCR test for the detection of MAP. Although fecal culture has been used as a standard method to detect MAP, this method can’ t detect all positive cases. Conclusions: Despite the fact that DNA extraction from blood samples is easier than that of fecal samples, replacement of fecal PCR with buffy coat PCR was recommended for the diagnosis of MAP. In addition, using fecal samples based on PCR for diagnosis of Johne’ s disease is considered more efficient.

Background: Activating the calcium-sensing receptor (CaSR), which regulates extracellular calcium, has been suggested to possibly decrease renin expression, thus playing a role in regulating the blood pressure via the renin-angiotensin-aldosterone system. Objectives: Considering this hypothesis, the aim of this study was to find the relationship between the gain-of-function variation of the CaSR gene, R990G (A < G), and coronary heart disease (CHD) risk factors and complications. Methods: The study sample consisted of 121 CHD patients and 105 healthy people from Turkish population. R990G function-gain mutation of the CaSR gene was analyzed by the real-time PCR method. Results: The AA genotype of CaSR R990G variation was associated with higher LDL-C, SBP, DBP and lower HDL-C in the CHD group compared to controls (P < 0. 001). The comparison by gender in theCHDgroup revealed increased systolic blood pressure inwomen with common AA genotype as compared to men (P = 0. 008) whereas in the control group, higher HDL-C and lower serum LDL-C (P < 0. 001 and 0. 031, respectively) were observed in women with AA versus men with AA. Higher blood pressure levels in women were not sustained in G allele carriers compared to men with G allele (P > 0. 05). Conclusions: The common AA genotype of CaSR R990G was found to be related to hypertension in CHD patients while showing a higher association with hypertension, especially in women. We also suggest that the CaSR R990G AA genotype may have diverse effects on serum lipids and that the genetic effect could differ by gender.

Glaucoma is a group of complex eye disorders characterized by increased intraocular pressure, optic nerve damage, and ultimately, optic nerve cupping, ending in irreversible blindness. The age of onset and the anatomy of the anterior chamber indicate that glaucoma consists of the categories of primary open-angle glaucoma, primary congenital glaucoma, and primary angle closure glaucoma. To date, many loci have shown to be associated with different types of glaucoma; however, the underlying cause of the disease remains ambiguous. Two main approaches exist to find new loci and genes associated with the disease: linkage analysis, which is applicable for large pedigrees with multiple affected and healthy individuals, and a genome-wide association study for large cohorts of sporadic cases. Careful patient selection is the first step in these kinds of genetic research. All subjects selected should be examined to identify the known gene mutations for the specific type of glaucoma. Subjects with no mutations in known genes are possible subjects of a genome-wide association study or linkage analysis.

Dear Editor, Hypoxia is a critical part of normal placentation characterized bypO2 < 20mmHgin the placenta during the 8th to 10th weeks of pregnancy (1, 2). Invasion of extravillous trophoblast cells (EVTs) to uterine spiral arteries creates the physiologic hypoxia by preventing maternal blood flow into the intervillous space. Decreased oxygen tension in the placenta is essential for the regulation of trophoblastic invasion, proliferation and differentiation, placental angiogenesis, and embryonic organogenesis (1-3)....

Background: Uterine leiomyomas (ULs) are benign tumors in the uterus that their growth and progression are stimulated by the estrogen hormone. In the current study, we aimed to determine if estrogen receptor  (ER ) polymorphisms could be used as the markers of the susceptibility to UL. Methods: The ER gene polymorphisms of 154 UL women and 186 controls were genotyped by PCR or PCR-RFLP methods. Results: The frequency of ESR PvuII T> C polymorphism genotypes did not differ among thewomenwith leiomyoma and controls. However, the frequency of ESR XbaI GG genotype was significantly higher than the frequency of AA genotype (27% vs 10%) in UL women and the UL risk was 4. 1 folds greater in women carrying GG genotype (P < 0. 0001). In the UL women, a higher frequency was observed for TG and CG haplotypes of ESR PvuII/XbaI polymorphisms compared to TA haplotype and these haplotypes showed 3. 2 and 1. 5 folds increases in UL risk (P = 0. 003 and P = 0. 04, respectively). The frequency of CA haplotype was higher in controls, and the CA haplotype might have a potential to protect against UL (P = 0. 04). Conclusions: The GG genotype of XbaI A> G polymorphism and TG and CG haplotypes of PvuII T> C / XbaI A> G polymorphisms could increase the risk of UL.

Background: Recently, findings have validated the significant role of DNA damage genes related to the pathogenesis of breast cancer (BC). The aim of the present investigation was to evaluate possibility roles of two common XRCC1 (rs25487; A > G) and ERCC1 (rs3212964; A > G) gene polymorphisms with the risk of sporadic BC. Methods: In a case-control study, consisting of 100 females identified with sporadic BC and 100 malignancy-free females as the control group. We used Tetra-ARMS Polymerase Chain Reaction (PCR) and PCR-Restriction Fragment Length Polymorphism (RFLP) methods to determine genotype frequencies of XRCC1 and ERCC1 genes. Results: The findings did not reveal a statistically significant difference in the genotype frequencies of XRCC1 and ERCC1 genes between the two groups (P > 0. 05). The frequency of G mutant allele for XRCC1 and ERCC genes was higher in cases compared to controls, while the difference between the groups was not statistically significant (P = 0. 202; OR: 1. 312; CI: 0864-1. 994), (P = 0. 352; OR: 1. 213; CI: 0. 808-1. 820). Conclusions: The current results provide evidence against the hypothesis that XRCC1 (rs25487) and ERCC1 (rs3212964) gene polymorphisms may be associated with a predisposition to sporadic BC.

Dear Editor, Diagnostic tests, which accurately diagnose the disease, play an essential role in the decision of clinicians regarding the type of treatment. Nevertheless, it is possible to evaluate the attribution of diagnostic tests, and choose the best possible one based on the results; to describe a test, sensitivity, specificity, and accuracy are extensively used. . . .