The Scientific Journal of Iranian Blood Transfusion
Year:2019 | Volume:16 | Issue:3|Papers Count:7

Background and Objectives: There is a circadian rhythm and diurnal variation in the redox capacity in blood plasma and there is a decrease in antioxidant capacity (TAC) during the RBCs storage in blood banking. So it is possible that donated blood, based on donation time, has a different antioxidant capacity and as a result a different biochemical response during storage. This study attempts to uncover this issue. Materials and Methods: In this experimental study, Twenty RBC units were collected from two donor groups in the morning (8-11am) and evening (5-8pm) and were stored for 3 to 42 days at 4 to 8 degrees Celsius. Glucose, Sodium, potassium, lactate, lactate dehydrogenase (LDH), pH, malondialdehyde (MDA), and total antioxidant capacity were measured by a commercial kit. Nitrate/nitrite was measured by Griess method. Results: The levels of nitrate/nitrite, MDA, and LDH in the evening group increased significantly when compared to the morning group (p < 0. 05). However, a decreasing trend in TAC, pH, Glucose, and sodium levels (p < 0. 05) versus an increasing trend in potassium, lactate, LDH, and MDA was seen in both groups during storage (p < 0. 05). Conclusions: This study showed that oxidative damage in donated blood in the evening was higher than in the morning during storage, which may reduce RBC survival. Therefore, more studies are required with more sample sizes to examine the effect of donation time on the RBC storage lesion.

Background and Objectives: Now day, using of stem cells and nanoparticles in the differentiation of stem cells is considered as a therapeutic approach. The purpose of this study was to synthesize and characterize nanocomposite polyacrylic polycarboxylic acid containing nanoparticles of biologically active glass 45S5 crushed and assessment effect of this composite on the propagation and growth of bone marrow derived mesenchymal stem cells in the external environment. Materials and Methods: In this experimental study, the bioactive glass nanoparticles were synthesized by planetary milling and coated on electrospun polyolactic acid nanofibers. The physicochemical and biological properties of the composite nanocarbon were evaluated by X-ray diffraction, scanning electron microscopy, MTT, acridine orange, and alkaline phosphatase assays. Results: The physicochemical properties showed that the structure of synthesis bioactive glass and the lactic acid poly lactic acid fibers were completely nano size and the uniform distribution of nanoparticles was well placed on the fiber. The cell analysis demonstrated the significant increase proliferation and differentiation of mesenchymal stem cells to bone cells. In the MTT assay, cell survival was expressed in the composite nanostructure after 7 days of cell culture (1. 96 ± 0. 2), while the control sample (0. 76 ± 0. 08). Conclusions: According to the results of this study, the composite nanoscale composite is not cytotoxicity effects and has biocompatible. It is useful in bone tissue repair.

Background and Objectives: Over ordering of blood in elective surgical procedures due to fear of bleeding and unavailability of sufficient blood can cause blood wastage and increase blood bank expenses. This study compared blood ordering and utilization after the introduction of maximum surgical blood ordering (MSBOS) in elective surgeries. Materials and Methods: This cross sectional study was carried out on one third of Shiraz hospitals (23 hospitals) between 2016 and 2017, Shiraz, Iran. The localized MSOBs was sent as a guideline to the hospitals. The mean number of blood requests, blood utilization, cross matches, cross match to transfusion ratio, and cross match expenses before and after implementing of MSBOS were compared with paired t test by SPSS-21 software. Results: The cross match to transfusion ratio was decreased after implementing MSOBS (2. 36 to 1. 6) (p < 0. 001). In addition, the mean numbers of blood usage and cross matches were also decreased after implementing of MSBOS (13. 2%, 42. 9%). The cost of preparing blood and cross matches also decreased (7. 67%, 43%). Conclusions: Implementation of MSBOS created a significant reduction in transfusion ordering and providing blood in emergencies. Updating of physician’ s knowledge, the revision of MSOBS according to blood usage in the hospitals must be performed for optimal blood management.

Background and Objectives: In Iran, Khorasan province is an endemic area for HTLV-1 virus. Considering the inability of serological tests to determine HTLV-1 in window period, their failure to confirm the indetermination results of western blot, and given the probability for HTLV-1 transfusion transmission, a SYBR green-based Real Time PCR was set to measure the HTLV-1 proviral load. Materials and Methods: In this experimental study, using a cloning method and drawing a standard curve, the Real-Time PCR test was run to determine the HTLV-1 proviral load. At first, genomic DNA was extracted from peripheral blood mononuclear cells. Then, the PCR product of the Tax gene was placed in a cloning vector and recombinant plasmid was diluted by drawing a standard curve and a real-time PCR test was conducted using SYBR Green method. Results: Cloning was performed using PCR product for tax gene, pTZ57/T vector, and E. coli (TG1 strain). Cloning accuracy was confirmed with Colony PCR and sequencing and used as the Real-Time PCR test standard. The standard curve was drawn with serial dilutions of recombinant plasmid containing Tax-1 gene. The slope of the standard curve was 3. 3 and R2 = 0. 99 which indicates the linearity and efficiency of the test reaction. Conclusions: Real-Time PCR method is an appropriate method to measure HTLV-1 proviral load.

Background and Objectives: Hemagglutination has limitations in identifying the phenotype of patients who have been recently transfused due to the presence of donor red cells (RBCs) in the patient’ s circulation. Kidd blood group is one of the most important blood groups in transfusion medicine and related antibodies are responsible for one third of delayed haemolytic transfusion reactions (DHTRs). In this study we developed a molecular procedure for detection of alleles responsible for Kidd blood group in thalassemia patients. Materials and Methods: In this cross sectional descriptive study, one hundred of alloimmunized thalassemic patients from Tehran Adult Thalassemic Clinic in 2017 were studied. The phenotype of samples was tested by routine serological methods. PCR-SSP, PCR-RFLP and DNA sequencing were used to determine the genotype of kidd blood group for these patients. Results: Discrepancies were found between the phenotype and genotype in 20 out of 100 cases. In 11(55%) cases phenotype was determined as Jk (a+b+) but genotype was JKA/JKA, in 7 (35%) cases phenotype was Jk (a+b+) while the genotype showed JKB/JKB, 1(5%) case had been phenotyped as Jk (a+b-) but it was genotyped as JKA/JKB, and 1(5%) case had been phenotyped as Jk (a-b+) but it was genotyped as JKA/JKB. Conclusions: Molecular techniques as a complementary method together with the results of serological methods can help resolve the problems caused by blood transfusion and bring about accurate determination of phenotype in thalassemic patients with multiple blood transfusions.

Background and Objectives: The main mission of blood centers is to provide safe and adequate blood products for patients. With the advancement of science and technology, the need for safe blood still has priority and no substitute for human blood is discovered. Participation in social activities such as voluntary blood donation should be provoked by different entities including public relations departments. The purpose of this study was to investigate the role of public relations departments in the motivation of citizens' participation in blood donation in Ardabil. Materials and Methods This cross-sectional study was conducted by multistage cluster sampling on 384 citizens over 18 years of age and using questionnaires in Ardabil. SPSS 22 data analysis software and regression data analysis were performed. Results: Donors aged between 18 and 60 years old participated in the study. Research findings showed that the mean rates of the role of public relations was 3. 66 (Deviation 0. 84), persuasive role 3. 72 (Deviation 0. 41), awareness role 3. 60 (Deviation 1. 46), problem-solving role 3. 11 (Deviation 0. 51), advisory role 3. 45 (Deviation 0. 48), the role of relationship management 2. 80 (Deviation 0. 57), the role of public education 2. 57 (Deviation 0. 63). According to the findings, the mean rates of the citizen participation are 3. 21 (Deviation 0. 33), conditional behavioral participation 3. 35 (Deviation 0. 51), solidarity-oriented participation 3. 36 (Deviation 0. 73), and development-oriented partnership 3. 22 (Deviation 0. 78). Conclusions: Dimensions of public relations (as persuasive, awareness, problem solving, advisory, relations management) in Iranian Blood Transfusion Organization has placed positive impact on the motivation of Ardabil citizens to participate in blood donation.

Background and Objectives: Obesity is associated with increased inflammatory factors such as fibrinogen and white blood cells. However, physically active people have lower levels of inflammatory factors. The aim of this study is to investigate the effects of eight weeks of aerobic exercise on fibrinogen level and blood cell number of obese girls. Materials and Methods: This study was conducted as semi-experimental and subjects were divided (BMI > 30) into two groups: aerobic training (AT, 12 subject) and control (Con, 12 subject). The former group did aerobic exercises for eight weeks (four sessions a week, each session 60 min with the max heart beat 65-75%). Blood samples were taken before and after the exercise program. T-test was used to compare the groups (using SPSS-16 statistical software and the significance level of p < 0. 05). Results: The results showed that aerobic exercise program had no significant effect on the fibrinogen level in the experimental (29. 19 ± 2. 46) and control (28. 08 ± 2. 59) groups and also on blood cell (p < 0/05). Osmolality was significantly reduced in the training group. Similarly, the dietary micronutrients and macronutrients analysis showed that between control and experimental groups throughout the training, there was no significant difference. Conclusions: Eight weeks of moderate intensity aerobic exercise had no significant effect on plasma fibrinogen and blood cells in obese girls. It seems that the exercise program should be associated with weight loss and reduction of fat mass to improve inflammatory markers associated with obesity.