Measurement of blood parameters is a diagnostic method to determine disease or health in aquatic organisms that can change by numerous factors. In this study 30 spotted catsharks Chiloscyllium punctatum, with similar characteristics of both sexes were caught from the northern estuary, of Persian Gulf. Blood samples were collected and parameters such as WBC, lymphocytes, monocytes, eosinophils, neutrophils and blood indices including MCV, MCH, MCHC were measured. Results indicated no significant difference between the measured parameters and both sexes (p>0.05). The highest count of lymphocytes and the lowest count of eosinophils were seen in males. White blood cells were higher in male than in female. The highest MCV was seen in male and the lowest MCHC was seen in female with no significant difference in both sexes. Differences in WBC and blood parameters in cat spotted shark with other marine organisms are discussed.