ISOLATION, IDENTIFICATION AND CLONING OF GROWTH HORMONE GENE FROM EPINEPHELUS COIOIDES IN E. COLI

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KHESHTZAR FATEMEH; ZOLGHARNEIN HOSSEIN; ARCHANGI BITA; RAJABZADEH EBRAHIM; GHASEMI AHMAD

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Journal Paper

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Journal: JOURNAL OF MARINE SCIENCES AND TECHNOLOGY Year:2016 | Volume:14 | Issue:4 Page(s): 0-0

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Title

ISOLATION, IDENTIFICATION AND CLONING OF GROWTH HORMONE GENE FROM EPINEPHELUS COIOIDES IN E. COLI

Author(s)

Keywords

GROWTH HORMONE

CLONING

EPINEPHELUS COIOIDES

PTZ57R/T VECT

Abstract

According to the role of the GROWTH HORMONE gene in the aquaculture, in this research CLONING GROWTH HORMONE coioides (Epinephelus coioides) was evaluated in vector pTZ57R / T. After PCR product purification by kit QIAquick Gel Extraction, GROWTH HORMONE gene was integrated intopTZ57R / T vector. Constructed vector was transferred in E. coli strain DH5α competent cells. The white colonies was recombinant bacteria, from that that plasmid extraction was performed. CLONING obtained conformed using with direct PCR and sequencing methods. GROWTH HORMONE cDNA from E. coioide has an open reading frame of 615 nucleotides and 204 amino acids respectively. The calculated molecular weight and predicted of isoelectric point of the GROWTH HORMONE protein were 014.23 kDa and 9.6 respectively. The results of this study showe that GROWTH HORMONE gene in pTZ57R / T vector successfully had been cloned and can be used to GROWTH HORMONE gene expression and protein production. The omparition of gene sequencing with GROWTH HORMONE gene from E. coioides in the GenBank was showed similarity between them.

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APA: Copy

KHESHTZAR, FATEMEH, ZOLGHARNEIN, HOSSEIN, ARCHANGI, BITA, RAJABZADEH, EBRAHIM, & GHASEMI, AHMAD. (2016). ISOLATION, IDENTIFICATION AND CLONING OF GROWTH HORMONE GENE FROM EPINEPHELUS COIOIDES IN E. COLI. JOURNAL OF MARINE SCIENCES AND TECHNOLOGY, 14(4), 0-0. SID. https://sid.ir/paper/695755/en

Vancouver: Copy

KHESHTZAR FATEMEH, ZOLGHARNEIN HOSSEIN, ARCHANGI BITA, RAJABZADEH EBRAHIM, GHASEMI AHMAD. ISOLATION, IDENTIFICATION AND CLONING OF GROWTH HORMONE GENE FROM EPINEPHELUS COIOIDES IN E. COLI. JOURNAL OF MARINE SCIENCES AND TECHNOLOGY[Internet]. 2016;14(4):0-0. Available from: https://sid.ir/paper/695755/en

IEEE: Copy

FATEMEH KHESHTZAR, HOSSEIN ZOLGHARNEIN, BITA ARCHANGI, EBRAHIM RAJABZADEH, and AHMAD GHASEMI, “ISOLATION, IDENTIFICATION AND CLONING OF GROWTH HORMONE GENE FROM EPINEPHELUS COIOIDES IN E. COLI,” JOURNAL OF MARINE SCIENCES AND TECHNOLOGY, vol. 14, no. 4, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/695755/en

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