Background and Objectives: It is of importance to find drought-tolerant tree species with the aim of reviving Zagros forests. There are no enough researches about the effect of nanoparticls on the woody plants especially on their physiological features, while most of the researches have focused on herbaceous and crop plants. Therefore, in the current research, we investigated the effect of nano Sio2 on wild pistachio (Pistacia atlantica subsp. mutica) seedlings to understand the plant reaction to tension in order to find a drought-tolerant plant for rehabilitation of Zagros forests. Materials and Methods: The experiment was a factorial experiment based on completely randomized design with 126 seedlings, three replicates, six treatments of nano Sio2 (control, 100, 300, 700, 1500, and 3000 mg. l-1) for 30 days and at three levels of drought stresses (control, every other day of irrigation; moderate, every four days of irrigation; and severe drought, with no irrigation). At the end, the physiological characteristics of chlorophyll a, b, total, caratoneid, carbohydrate, proline content, and antioxidant enzymes of guaiacol peroxidase and catalase were analysed in seedlings leaves. Results: The results showed that using nano Sio2 increased different kinds of chlorophylls, caratoneid, proline content, carbohydrate, and antioxidant enzymes under drought stress. In fact the seedlings reacted differently to nano Sio2 treatments that the highest amount of carbohydrates and catalase were observed in 700 mg. l-1; chlorophyll a, b, total and caratoneid in 3000 mg. l-1, and proline and peroxidase in 300 mg. l-1. Furthermore, the different levels of drought stress reduced chlorophylls and caratoneid; while increased soluble carbohydrates, proline, and antioxidant enzymes. Conclusion: It can be concluded that the most physiological traits were increased under drought stress and the Sio2 nanoparticles had positive effect on traits of different types of chlorophyll and carotenoids. Concentrations of 300, 700 and 3000 mg. l-1 had a better effect on many of the physiological traits than the others. Reducing chlorophylls and carotenoids and increasing carbohydrate, proline and antioxidant enzymes are occurred in plants to tolerate the drought stress. Due to the lack of basic information about the resistance mechanism in endemic tree species from Zagros forests in response to nanoparticles, this study will be a great assistance to develop nano technology in wooden plants. It is suggested to study the effect of Sio2 nanoparticles for longer periods or on some more species that could be a suitable way to find better results. These results may lead into economical production of drought tolerant seedlings.