To study the genetic diversity based on agronomic, physiological and drought tolerance indices of 20 genotypes of durum wheat, an experiment was carried out under stress and non-stress conditions in research field and physiology laboratory of agricultural and natural resources campus, Razi University, Kermanshah, Iran, in 2013-2014 cropping season. Experimental design was randomized complete block design with three replications under stress and non-stress conditions. Analysis of variance for different agronomic, physiological and biochemical traits showed that the effect of genotype was significant on water content in cut leaves, water use efficiency, stomata conductance, chlorophyll b, total chlorophyll, grain yield, activity of catalase, peroxidase and superoxide dismutaseenzymes. Mean comparison showed that the genotypes 20, 5, 3, 1, 12 and 13 had the highest values of traits and indices. For example, grain yield of 535. 3 g. m-2, relative water content of 73. 2%, the photochemical efficiency of photosystem II (Fv/Fm) (0. 769) and the enzyme activity of superoxide dismutase (2. 11 U. mg-1). The correlation analysis for drought stress conditions revealed significant positive correlation between grain yield with chlorophyll b, and between total chlorophyll as well as relative water content and photochemical efficiency of photosystem II. Significant negative correlation was observed between water content of cut leaves and the activity level of superoxide dismutase enzyme. There was a significant positive correlation between chlorophyll a content with chlorophyll b and total chlorophyll contents as well as between chlorophyll b and total chlorophyll. The GGE-BIPLOT analysis identified genotypes 5, 6, 20, 9, 2, 3, 10, 4, and 19 as superior genotypes for grain yield and yield components. Also, the use of physiological and biochemical indices as well as genetic diversity assessment using GGE biplot are very valuable tools for identifying highperformance genotypes with yield stability for drought tolerance. This information is of high importance in applied durum wheat breeding programs for slection of parents for cropssing and development of drought tolerant cultivars.