Entrepreneurship is a multi-faceted phenomenon, which, on the one hand, is affected by all economic, social, cultural and political dimensions and, on the other hand, affects these dimensions. Therefore, the development of entrepreneurship depends on the different aspects of the angles and the various aspects that necessitate the application of the ecosystem approach. This research was essentially aimed at examining the existing and desired status of the components of the ecosystem of entrepreneurship in knowledge companies. The statistical population of the research consisted of the members of knowledge societies in Kermanshah and Hamedan provinces. Using the Pontoon chart, 335 of them were selected through a sampling procedure. The main tool of the research was questionnaire, the reliability of the questionnaire was calculated by sequential alpha coefficient (88%-86% = α ). The collected data were analyzed using SPSS software. The results showed that the greatest gap between the existing and the optimal level of the dimensions of the business ecosystem in the knowledge-based companies, in terms of support, financially, is visible and the smallest gap in the human resources, as well as the components related to the market and financial dimensions according to the amount z have the most difference.