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Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    279-283
Measures: 
  • Citations: 

    1
  • Views: 

    151
  • Downloads: 

    74
Abstract: 

Background and Aims: Hepatitis C virus is a major global health problem that primarily affects the liver which lead to liver cirrhosis, liver failure and hepatocellular carcinoma. The aim of this report was to investigate the genotypes of HCV in patients on regular hemodialysis in Iraq. Materials and Methods: A cross sectional study was conducted to investigate the genotypes of HCV in patients on regular hemodialysis in 7 centers across Iraq. A total of 255 subjects with positive anti-HCV antibodies were recruited in this study. Results: To confirm the positivity, RT-PCR was performed. Amongst all samples, 31% (79/255) was positive by RT-PCR. Then, we genotyped all the RT-PCR positive samples, it was found that 39/79 (49. 4 %) of our samples were typed as HCV genotype 1 including 26 samples (66. 7%) as HCV 1a and 13 samples (33. 3%) as HCV 1b. HCV genotype 4 was found in 34/79 (43%) of the samples while 6/79 (7. 6%) of the samples typed as HCV 3 Conclusion: HCV genotype 1 was the most prevalent genotype followed by genotype 4. Further population-based study is required to investigate the prevalence of HCV genotypes.

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    284-293
Measures: 
  • Citations: 

    0
  • Views: 

    99
  • Downloads: 

    47
Abstract: 

Background and Aims: Periodontal diseases are very common among the people of all ages and also a proof of oral hygiene maintenance practice. A variety of microorganisms are found in human oral cavity which can often cause periodontitis, cavities, gingivitis, mineralization of tooth and other oral diseases. Current study was carried out to determine which microbes are responsible for dental caries among the patients in Dhaka along with control with no dental disease. Materials and Methods: After observing the symptoms of dental caries like tooth pain, bleeding gum, bleeding while eating and brushing, weakening of teeth, mobility of teeth, swelling of gum etc.; dental plaque samples were collected and diluted in thioglycollate broth. The diluted sample was then transferred to a series of specific media including Mitis salivarius Agar (MSA), BD LBS Agar, Actinomycetes Isolation Agar, NOS Spirochete medium, P. GING (Porphyromonas gingivalis Agar) and VCAT and incubated at 37oC to isolate Streptococcus spp., Lactobacillus spp., Actinomycetes spp., Treponema spp. and Capnocytophaga spp. One sample was taken from a healthy patient as a control test harboring no pathogenic microbial growth. Results: According to current study, the most common microbes which can initiate these dental diseases include Streptococcus mutans, Lactobacillus spp., Capnocytophaga spp., Porphyromonas gingivitis, Actinomycetes spp. etc. After detecting the mentioned microorganisms, different procedures were prescribed for the patients including scaling, root cannel, surgical removal of teeth (tooth extraction) etc. Conclusion: Oral hygiene should be maintained not only by the patients, but also by every person to lessen the risk of dental disease as well as the early detection of it to prevent the loss of tooth.

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    233-250
Measures: 
  • Citations: 

    0
  • Views: 

    251
  • Downloads: 

    201
Abstract: 

Background and Aims: The incidence of foodborne infectious diseases has been stable and even increased in many countries. Improper use of antibiotics due to the prevalence of microbial diseases has caused drug resistance. So nanotechnology has many attractive applications in the food industry, such as food preservation and food quality control. By the reason, the absorptive and antibacterial features of copper oxide nanoparticles in combination with magnesium oxide nanoparticles in delete of bacteria were investigated. Materials and Methods: The antibacterial activities of CuO NP in combination with MgO NP against Escherichia coli and Staphylococcus aureus in culture media and fruit juice (mango, pomegranate, and peach) by agar diffusion and colony count method were explored. Electron microscopy was used to characterize the morphological characteristics of the bacteria tested after treatment with CuO and MgO NPs. Results: The results of one-way ANOVA by 95% confidence showed that CuO and MgO NPs have antimicrobial activity on E. coli and S. aureus. An effect of synergism was observed when combining CuO and MgO NP. Electron microscopy photographs showed that treatment with the combination of MgO and CuO caused damage to the cell membrane. As a result, the leakage of intracellular contents kills the bacteria. Conclusion: The combination of CuO and MgO nanoparticles can successfully control the growth of E. coli and S. aureus in liquid and juice medium. So, this combination treatment can reduce the required amount of CuO and MgO nanoparticles during the pathogen control process in the food industry.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    251-265
Measures: 
  • Citations: 

    0
  • Views: 

    417
  • Downloads: 

    204
Abstract: 

Background and Aims: The use of ethanol-temperature-resistant Acetobacter strains to produce vinegar on an industrial scale is important due to their sensitivity to high ethanol concentration as substrate and high energy consumption for acetator cooling. The aims of this study were to isolate and identify high temperature and ethanol resistant Acetobacter strains as starter for production of vinegar. Materials and Methods: The banana alcoholic extract was transferred to the fermenter medium and after 24 h incubation at 30° C, colonies with transparent zone were purified as acetic acid bacteria and examined macroscopically and microscopically. The resistance to high temperature in constant ethanol and time as well as resistance to high ethanol in constant temperature and time were investigated. Results: The studies of AAB isolate grown in the Carr medium showed that it was a Acetobacter strain. According to the single-factor optimization, this species was able to grow in a Carr medium containing 9% ethanol at 40° C after 72 h. Conclusion: This is the first report of an AAB isolation from banana in Iran. This bacterium, as a new resistant strain to high levels of ethanol and temperature, was identified as Acetobacter ghanensis KBMNS-IAUF-6 and its 16S-rDNA sequence was deposited in GenBank, NCBI, under the accession number of MK968570. This new strain can be suggested as a high temperature and ethanol resistant strain for producing banana vinegar on a semi-industrial and industrial scale.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    266-278
Measures: 
  • Citations: 

    2
  • Views: 

    400
  • Downloads: 

    220
Abstract: 

Background and Aims: Co-word analysis, based on Co-occurrence, as one of the important techniques of Scientometrics and bibliometrics, enables the analysis of the content of scientific documents of the specific Research Area. The purpose of the present study is visualize HPV clusters relationships and thematic trends in the world. Materials and Methods: The research type is an applied one with analytical approach and it has been done using co-word analysis. The population of this study consists of articles’ keywords indexed during 2014-2018 in the Web of Science (WoS) in HPV subject area. The total numbers of the retrieved and analyzed keywords in this study were 13249. Some software like SPSS, UCINET and VOSViewer were used for data integration and analysis. Results: The findings showed that the keyword “ CERVICAL CANCER” have had the highest frequency and with “ CERVICAL INTRAEPITHELIAL NEOPLASIA” and they were co-word couples. The results of the strategic diagram showed that the most clusters in HPV placed in third area of strategic diagram, it means these subjects (clusters) were emerging or declining. Conclusion: Co-word analysis is suitable method for discover and visualize different sciences and their prominent patterns, hidden relationships and thematic trends research’ subject areas. The results of these analysis and findings of such researches will help research policy makers.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesDownload 220 مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesCitation 2 مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesRefrence 0
Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    294-301
Measures: 
  • Citations: 

    0
  • Views: 

    404
  • Downloads: 

    287
Abstract: 

Background and Aims: A major problem in the treatment of Pseudomonas aeruginosa infections is the emergence of strains with multiple resistances (MDR). The aim of this study was to identify virulence genes lasB, toxA, algD, exos in P. aeruginosa isolated from human and animal using Multiplex-PCR method and determination of antibiotic resistance. Materials and Methods: This cross-sectional study was performed on the 120 non-repetitive samples, including, 60 clinical samples of human and 60 animal samples collected from Tehran, Iran. Antibiotic susceptibility test was performed by disk diffusion test. The multiplex-PCR method was performed to identify various virulence genes. Results & Conclusion: The highest resistance rate was related to amoxicillin and amikacin in the both types of samples. lasB and exoA genes were the most prevalent virulence determinants in the human and animals samples, respectively. The results of antibiotic susceptibility test showed that the resistance in human strains was far higher than animal and this could be the result of arbitrary administration of the drug. The lasB gene in human specimens and the exoA gene in animals play an important role in the development of the disease.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    302-309
Measures: 
  • Citations: 

    0
  • Views: 

    308
  • Downloads: 

    212
Abstract: 

Background and Aims: Urinary Tract Infection (UTI) is one of the most common bacterial infections, and several studies have reported the prevalence of UTI caused by ESBL-producing E. coli strains in different cities of Iran. The aim of this study was to evaluate antibiotic resistant UTI factors in outpatients and determine the prevalence of ctx-m gene in ESBL-producing E. coli strains of Bonab (East Azerbaijan, Iran). Materials and Methods: In 2015, 266 clinical isolates of E. coli were collected from clinical laboratories in Bonab. Phenotypic screening and confirmation tests for extended-spectrum beta-lactamases (ESBLs) were carried out using disk diffusion (Kirby Bauer) method. All of the ESBL producing isolates were tested by PCR using specific primers. A total of 10 PCR products were randomly selected and purified and subsequently sent for sequencing. Results & Conclusion: The maximum resistance was seen regarding ampicillin (67. 3 %) and the maximum sensitivity was seen regarding imipenem (92. 5%). In this study, 45 % of isolates were multidrug resistance, which showed at least resistance for three antibiotics. Out of 154 isolates, 58 (37. 7%) isolates were ESBL producers which 94. 8% of isolates contained ctx-m gene. The prevalence of the ctx-m gene, as in other parts of Iran and the world, is high in Bonab. For the treatment of infections suspected to be an ESBL producer, appropriate antibiotics should be selected, and strains that have decreased their sensitivity to ceftazidime, cefotaxime, and cefetoxin should be considered for ESBL genes.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    13
  • Issue: 

    4
  • Pages: 

    310-320
Measures: 
  • Citations: 

    6
  • Views: 

    358
  • Downloads: 

    179
Abstract: 

Background and Aims: Medicinal herbs are used due to fewer side effects for produce of natural drugs in the pharmaceutical industry. The aim of this study was investigation of antibacterial and antioxidant activity of Citrus medica skin extracts against 10 human pathogenic bacterial and investigation of secondary metabolites in vitro. Materials and Methods: Color and white Skin of Citrus medica under the supervision of Citrus Research Center experts were collected from Ramsar City, Mazandaran Province, Iran in 2014. Antibacterial activity by agar well diffusion method, total phenolic content by Folin-Ciocalteu method, total flavonoid by aluminum chloride and antioxidant properties by DPPH method were investigated. Phytochemical investigation of secondary metabolites was performed. Results & Conclusion: The highest inhibitory activity was observed on methanol extract of color skin on Bacillus cereus bacteria. IC50 methanol extract of color, white skin and ascorbic acid were calculated 0. 1505, 0. 1738 and 0. 1095 mg/mL respectively. The total phenolic content of color and white skin was 109. 5 and 105. 6 (mgGAE/g) and the total flavonoid content was 3. 53 and 3. 02 (mgQ/g), respectively. The presence of alkaloid in methanol extract of color skin and the presence of saponin and tannin in methanol extract of white skin was confirmed. Due to antibacterial properties of this medicinal plant can be used in produce antibiotics controlling of human pathogenic microorganisms.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesDownload 179 مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesCitation 6 مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic ResourcesRefrence 0
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