An interregional input-output analysis can illustrate the full picture of trade between regions at the economic sect oral levels. This analysis is due to the availability of an interregional input-output table (IRIO). Since the IRIO tables are not provided by an official institution in Iran, in the regional input-output literature, it is necessary to use no survey methods. In this regard, the main objective of this paper is to estimate the trade between two major provinces, Tehran and Isfahan in order to prepare a two-region IRIO table that the sum of which is not the national economy. This table can measure not only the trade between the regions but also prepare the international trade in the above areas. The main data bases of the research are: The National Statistical Input-Output Table of 1390, and the regional accounts of Tehran and Isfahan provinces at the level of 72 economic sectors from the Statistical Center of Iran, which have been aggregated in to 9 sectors to describe the results. Findings indicate that, Isfahan province’ s imports from Tehran is 5 times of Tehran province’ s imports from Isfahan. The greatest value of sect oral import of the two regions belongs to other manufacturing sectors. The value of total trade between Tehran and Esfahan is 546 and 551 trillion rails respectively. The highest international sect oral imports and exports of the provinces of Tehran and Isfahan are related to the manufacture of various chemicals, metals, minerals, machinery, equipment and tools sector and other manufactures. The calculation of the import coefficient shows that the highest import coefficient of Tehran province from Isfahan is 0. 039 and the highest import coefficient of Isfahan province from Tehran is 2. 439 and both are in the section "other manufacture" of industry.