Zinc (Zn) is one of the micronutrients for plants and its deficiency is common in calcareous soils. Organic matter plays a key role in availability and transformation of soil Zn. In order to study the effect of leonardite on adsorption behavior and chemical forms of Zn in a sandy loam soil, an experiment was conducted in a completely randomized design (CRD) with three levels of leonardite (0, 2 and 5%) with three replications. The soil was mixed with 0, 2 and 5% of leonardite and incubated at field capacity (FC) at 25± 1 ° C for two weeks. After two weeks, adsorption experiments carried out with different initial concentration of Zn (0, 30, 90, 120, 150, 200, 250 and 300 mg L-1 Zn) with 0. 01 M CaCl2 as a background solution, and Zn-extractable by DTPA method and Zn distribution by modified Tessier sequential extraction method were determined. Among the adsorption equations, the Froundlich equation (with higher R2 and lower SE) was better fitted to experimental data compared to the Langmuier, Temkin and Dubinin-Radushkevich equations, the sorption capacity factors (qmax, B, KF, qD) and sorption energy factors (KT, KL, 1/n) decreased with application of the leonardite. The sorption energy parameter (E) of Dubinin-Radushkevich isotherm indicated that the Zn adsorption process was physical. The application of the leonardite increased the exchangeable, carbonate and organic matter forms but decreased the oxide and residual forms. The MF Values increased with increasing the leonardite levels, demonstrating an increase in the mobility of the Zn in the studied soil. It was concluded that addition of the leonardite in soil lead to transformation of zinc from insoluble forms into more soluble and increased zinc bioavailability.