Journal of Inflammatory Diseases
Year:2014 | Volume:18 | Issue:4 (75)|Papers Count:13

Background: Coronary artery disease (CAD) is the most important cause of death around the world. C-reactive protein (CRP) is one of the indicators of systemic inflammation and is an effective factor in the development of inflammatory response in acute coronary syndrome.Objective: The aim of this study was to determine the association of quantitative CRP with acute myocardial infarction.Methods: This case-control study was carried out on 90 patients with diagnosis of acute myocardial infarction as case group and 90 healthy subjects as control group in Birjand Valiasr hospital during 2013. Highly sensitive CRP (hs-CRP) level was measured. The association of hs-CRP and cardiovascular risk factors was assessed. Data were analyzed using Chi-square test.Findings: The mean age was 58.6±13.9 and 57.4±15.4 years in the case and control groups, respectively. 70% of the case group and 66.7% of the control group were male. 76 patients (84.4%) in the case group and 56 subjects (62.2%) in the control group had high hs-CRP and the difference was statistically significant. The association of hs-CRP with gender and age was not statistically significant in two groups.Conclusion: With regards to the results, there is a relationship between increase in hs-CRP and acute myocardial infarction.

Background: Because of the relationship between sesame oil consumption and reduced cardiovascular morbidity and mortality rate, research on dietary intake of sesame oil is necessary.Objective: The aim of this study was to determine the effects of dietary supplementation with virgin sesame oil (VSO) on lipid profile in rats.Methods: This experimental study was conducted in 28 adult male rats weighing 240-350 g. The experimental groups were fed with a diet supplemented with 0.25, 0.5 and 0.75 ml/kg/day VSO via gastric gavage for 30 days and the control group received saline. Serum levels of cholesterol, triglycerides, HDL and LDL were measured. Data were analyzed using one way ANOVA with LSD as post-hoc test.Findings: Serum cholesterol, triglycerides and LDL levels were significantly decreased in the experimental groups compared to the control group. HDL was increased in a dose dependent fashion, but the difference was statistically significant for the 0.5 and 0.75 ml/Kg/day groups compared to the control group.Conclusions: With regards to the results, virgin sesame oil supplementation decreased serum lipids that is probably due to the effects of antioxidants and unsaturated fatty acids.

Background: S100A8 as a subunit of calprotectin heterodimer plays a role in inflammatory processes and cancer.Objective: The aim of this study was to express recombinant S100A8 and to evaluate Ca effect on its tertiary structure.Methods: This experimental study was performed in Qazvin University of medical sciences, 2013. Recombinant S100A8 subunit was expressed in pET15b, E. coli BL21 (DE3) system as a his-tagged protein. The protein purification process was accomplished under both native and denaturing conditions using Ni-NTA column and different concentrations of imidazole. The expression and homogeneity of recombinant protein was analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The tertiary structure of S100A8 was studied in the absence and presence of Ca using fluorescence spectrometry.Findings: The highest expression of S100A8 with apparent molecular weight of 8 kDa was found in denaturing conditions. In the purification process, the most purified S100A8 was seen with a gradient of 150 and 200 mM imidazole. The maximum fluorescence emission of S100A8 was observed at 330 nm in the presence and absence of calcium. Emission intensity was decreased in the presence of different concentrations of calcium.Conclusion: Changes in tertiary structure of S100A8 subunit in the presence of calcium may affect the protein function and may contribute to understand the role of this protein in cancer and inflammatory processes.

Background: Human group A rotaviruses are the most common viral agents of severe diarrhea in children and infants worldwide.Objective: The aim of this study was to determine different genotypes of rotaviruses in children with acute gastroenteritis in Karaj.Methods: This cross- sectional study was performed on 200 stool specimens from under 5 years old children with acute gastroenteritis referred to Bahonar hospital in Karaj from September 2011 to December 2011. Rotavirus contamination and different genotypes were determined by semi-nested multiplex Reverse Transcription Polymerase Chain Reaction RT-PCR.Findings: Rotavirus contamination was found in 27 samples (13.5%). P and G genotypes prevalence was found to be: P[8]: 44%, P[4]: 4%, P[6] P[8]: 4%, G1: 15%, G2: 8%, G4: 8%, G9: 15% and G4G9: 8%.Conclusion: With regards to the results, P[8], G1 and G9 were the predominant genotypes. Lack of amplification of segments related to VP7 protein and different genotypes in considerable percentage of the positive samples suggest the necessity of using other primers and further molecular studies in order to select and design new primers.

Background: Depression and anxiety disorders are among the most common psychiatric disorders in general population. Chronic diseases like osteoarthritis can accompany with increased level of anxiety and depression and may negatively affect patients’ mental health by related chronic pain and disability.Objective: The aim of this study was to determine the association of anxiety and depression with osteoarthritis.Methods: This case control study was conducted in 100 patients with osteoarthritis referred to the rheumatology clinic of Avicenna hospital and 100 healthy controls without any history of known psychiatric disorders or taking medication during 2013. Depression and anxiety were assessed by the Beck Depression Inventory and Ketel Anxiety questionnaire, respectively. Demographic information and pain years were measured using a questionnaire. Data were analyzed using Chisquare test, student T-test and Pearson’s correlation coefficient.Findings: 66% of the patients with osteoarthritis and 58% of the controls were diagnosed to have depression and the difference was not statistically significant. 7% of the case group and 2% of the control group had major depression. Frequency of anxiety was 45% in the patients with osteoarthritis and 42% in the controls. 17% of the case group and 12% of the control group had severe anxiety.Conclusion: With regards to the high frequency of depression and anxiety among patients with osteoarthritis and their impact on patients’ compliance, early diagnosis and treatment of them are necessary.

Background: Nocturnal enuresis is one of the most common disorders of childhood after allergic disorders.Objective: The aim of this study was to compare parent functioning and behavioral problems in children with and without primary nocturnal enuresis.Methods: This case-control study was conducted in 292 children aged 5-15 years old (146 with primary nocturnal enuresis and 146 without primary nocturnal enuresis) referred to Amir Kabir hospital in Arak during 2011-2012. Data were collected by Child Behavioral Check List (CBCL) and Global Assessment Functioning (GAF) questionnaire for parents. Data were analyzed using Chi-square test and T-test.Findings: In both groups of children with and without primary nocturnal enuresis, the most common problems were mental, anxiety-depressive and the least common problems were attention deficit-hyperactivity and physical. Differences in internalizing, externalizing, anxiety-depressive, isolation-depressive and affective-behavioral problems were statistically significant between the two groups; but differences in parent functioning were not statistically significant.Conclusion: With regards to the results, parental intervention for early treatment of children with primary nocturnal enuresis is necessary to prevent future behavioral problems.

Background: Rapid response nursing team assesses patients at an earlier stage of clinical deterioration and reduces mortality rate.Objective: The aim of this study was to determine the effect of setting up rapid response nursing team on cardiopulmonary arrest and unexpected death in patients admitted to emergency department.Methods: This randomized clinical trial was performed on 714 patients in the emergency department of selected hospital affiliated to Alborz University of Medical Sciences during 2013. Patients were randomly allocated to two groups. Rapid response nursing team consisted of a critical care nurse and an anesthesia technician that attended in the emergency department during work shifts 24 hours a day. The control group was cared by the department staff as routine. The intervention group was cared by the rapid response nursing team when the patient condition was deteriorating and after a careful evaluation. Results of the interventions were recorded for both groups. Data were analyzed using T- test, Chi-square test, and Fisher’s exact test.Findings: There was no significant difference between the two groups in terms of age and gender. There were significant associations between setting up rapid response nursing team and transfer rate to the intensive care units and mortality rate outside of the intensive care units.Conclusion: With regards to the results, setting up rapid response nursing team was effective in reducing transfer rate to the intensive care unit, mortality rate and cardiopulmonary arrest.

Background: Aflatoxins are forms of mycotoxins that cause aflatoxicosis in both domestic animals and human population and are both teratogenic and carcinogenic. Aflatoxin M1 (AFM1) is a hyroxylated metabolite of aflatoxin B1 which is produced in the liver of animals and can be excreted in the milk.Objective: The aim of this study was to review systematically previous studies of the aflatoxin contamination in the products of animal origin in Iran.Methods: This systematic review was conducted on all published studies between 2003 and 2013 that examined and quantified aflatoxin contamination of products of animal origin using HPLC and ELISA methods in different institutes and research centers in Iran. Pub med, Science Direct, SID and magiran databases were searched.Finding: 100% of milk samples were contaminated by AFM1 in Iran. The level of AFM1 contamination in the pasteurized milk of Tehran was less than other cities. The level of AFB1contamination was low in meat and other products of animal origin.Conclusion: High levels of aflatoxin in milk and dairy products indicate the necessity of revising national standards in products of animal origin. Using appropriate methods to neutralize this toxin in the dairy industry should be considered.

Radicular cysts associated with deciduous teeth are rare. This report discusses a case of radicular cyst associated with a right mandibular second deciduous molar in a 12 year old boy referred for delayed eruption of second permanent premolar and rapid growth of the cyst after pulpotomy. The treatment included enucleation of the cyst and removal of the associated tooth.

This descriptive study was conducted to assess the operating room safety standards of all teaching hospitals (Kosar, Booali Sina, Shahid Rajaee, Qods and Velayat) in Qazvin during 2012. The measurement tools were hospital information sheet and the operating room safety checklist that were completed by observation and interview with appropriate authority. The operating room of Kosar hospital was unsafe while operating rooms of other hospitals were safe. It seems that continuous assessment and fixing potential problems of the operating room safety standards by all personnel make progress and continuous quality improvement in health services.