The present study aimed at investigating the mediating role of mindfulness in the relationship between depression, anxiety, stress and psychological well-being. Current study is a descriptive study. A total of 198 (137 female and 61 male) participants completed the paper-and-pencil form of Depression, Anxiety, and Stress Scale (DASS), Mindfulness Attention Awareness Scale (MAAS) and Ryff Psychological Well-being Scale. The mediating role of mindfulness was explored using the PROCESS v3. 0 plugin for SPSS. The results revealed that mindfulness significantly (p<0. 01) mediated the relationship between 1) anxiety and total well-being, 2) anxiety and mastery as well as depression and master, and 3) all three independent variables and self-growth. Data also showed that, regarding some emotional disturbances (i. e., anxiety), trait mindfulness could prove similarly effective with both low and high emotionality, while with respect to other difficulties (i. e., depression) it will only foster psychological well-being when negative affectivity level is only low or moderate.