High molecular weight glutenin subunits variation was studied at Glu-1 loci in 86 breeding lines and cultivars of bread wheat of lran, using SDS-PAGE method. A total of 14 alleles and 31 allelic composition were detected. An already reported rare subunit pair" 2***+12'" at Glu-D1 locus was found in Sorkhtokhm, and Boulani which are landraces of Sistan and Bluchestan province of Iran and cv. Kavir which is a Sorkhtokhm derivative. The total average of Glu-1 quality scores of these lines and cultivars was 7.6 of 10 and showed that the HMW-GS compositions had generally good quality. Cultivars Inia, Maroon, Sisson, Zagros, Pastour, Tajan and N-75- 11, N-75-15, C-79-16 and C-79-18 lines showed quality scores equal to ten. However, Alamoot, Shahriar, Hamoon cultivers and C-73-18, C-73-20, C-75-5, and C-78-I8 lines showed quality scores between four to five. The quality of former and later lines/ cultivars was considered the highest and lowest in this study, respectively. The results in this study provide a genetic estimation of quality of bread wheat lines/ cultivars in Iran in comparison to those from other countries.