In order to investigate the physiological and morphological traits of seven autumn sugar beet (Beta vulgaris L.) cultivars (Jolge, Palma, Giada, Monotunno, SBSI1, Suprema and PP8), under freezing stress, a study was carried out as a factorial experiment (7×10) based on randomized complete block design with three replications in college of Agriculture of Ferdowsi University of Mashhad. The plants at seedling stage were exposed to ten freezing temperatures (0, -2, -4, -6, -8,-10, -12, -14, -16 and -18oC). Then, electrolyte leakage percentage, yield of quantum efficiency (Fv/Fm), net photosynthesis, leaf number, leaf area, root length, root diameter and survival percentage indices were measured. Results showed that survival and electrolyte leakage percentage in Monotunno cultivar was 88 and 26 %, respectively, which showed superiority over other cultivars. The Minimum and maximum value of LT50su (-16.9 and -15.2oC) and also Fv/Fm (0.7 and 0.59) were observed in Monotunno and SBSI1 cultivars, respectively. Results indicated a strong and negative correlation between Electrolyte leakage (EL) and survival percentage (r = -0.65***) and also among EL with other traits, whereas survival percentage had a strong and positive correlation with leaf number (r = 0.88***) and root length (r=0.87***). A significantly positive correlation (r = 0.97***) between survival percentage and Fv/Fm ratio and also a negatively significant correlation between Fv/Fm and LT50su (r = -0.85***) and LT50el (r = -0.84***) showed the cultivars with high survival percentage and also low electrolyte leakage have high Fv/Fm compared with sensitive cultivars. In cold tolerant cultivars with reduction of EL% the LT50el and LT50su indices decreased significantly, but correlation between LT50el and LT50su was significantly positive (r= 0.75*).