Introduction: Treatment of gingival recession and root coverage of denuded roots is one of the main goals of periodontal treatment.The purpose of this study is evaluation of two root conditioning techniques: EDTA (PH =7,24 %) and citric acid (PH=1) in free gingival graft procedure.Methods: In this clinical trial study, 16 patients with 24 class I, II gingival recession (Miller classification) on the buccal aspect of anterior teeth were selected. Hygienic phase of periodontal treatment carried out for all patients. and then patients divided into two groups: 12 teeth on the test group; FGG and EDTA conditioning, and 12 teeth on the control group; FGG and citric acid conditioning.Clinical examination and measurements consisting of oral hygiene, recession depth (RD) and clinical attachment level (CAL) before, 6 weeks, and 3 minutes after surgery. The results were analyzed by using Mann Whitney test and significant level of p<0.05 Results: Both test and control group shows significant reduction in RD three months after operation in comparison with pre-surgical state. The result shows that test and control groups didnt have significant differences in percent of root coverage, PD, CAL. KW, RW, and RD 3 months after surgery.Conclusion: Using EDTA causes significantly higher amount of keratinized width compared to citric acid conditioning.