Background and Aim: Endothelial function deteriorates with aging. On the other hand, exercise training improves the function of vascular endothelial cells. Endothelin-1 (ET-1), which is produced by vascular endothelial cells, is a potent constrictor and therefore, has been implicated in regulation of vascular tonus and progression of atherosclerosis. hypothesized that plasma ET-1 concentration increases with age, even in healthy adults, and that lifestyle modification (i.e., exercise) can reduce plasma ET-1 concentration in previously sedentary old adults.Materials and Methods: measured plasma ET-1 concentration in healthy young (21-28 yr old), healthy middle-aged (31-47 yr old), and healthy old (61–69 yr old) women. The plasma level of ET-1 increased significantly with aging (1.02±0.08, 1.33±0.11, and 2.90±0.20 pg/ml in young, middle-aged, and old women, respectively). Thus plasma ET-1 concentration was markedly higher in healthy old women than in healthy young or middle-aged women (by 3-and 2-fold, respectively). In healthy old women, we also measured plasma ET-1 concentration after 3 month of aerobic exercise (cycling on a leg ergometer at 80% of HRR for 30 min, 5 days/wk).Results: Regular exercise significantly decreased plasma ET-1 concentration in the healthy old women (2.22±0.16 pg/ml, P<0.01) and also significantly reduced their blood pressure.Conclusion: The present study suggests that regular aerobic-endurance exercise reduces plasma ET-1 concentration in old humans, and this reduction in plasma ET-1 concentration may have beneficial effects on the cardiovascular system.