The objective of the research is to validate the AOT for estimation of VO2max in healthy males and females. Subjects included 55 males and 50 females selected by purposeful stratified sampling based on health and age criteria.Subjects participated in both field and treadmill tests during a one week period.Age subgroups were 17-20, 21-25, 26-30, 31-40 and +41 consisting of at least 10 volunteers. The criterion measures were directly obtained from gas-analyzer outputs during a treadmill workout using AOT protocol. For men mean (±SD) of age, weight, height, maximal heart rate, BMI and VO2max were 31 (±11yrs), 74 (±12kg), 175 (±6cm), 191 (±11beat), 4.06 (±3.9kg/m2), 38 (±6ml/kg/min), respectively. The same measures for women were 9 (±10yrs), 62 (±11kg), 161 (±5cm), 191 (±12beat), 23.90 (±4.0kg/m2), 33 (±8ml/kg/min), respectively.The study showed that increase in BMI and aging results in decrease in maximal heart rate and VO2max. Compared to similar studies, produced formulas are scientifically acceptable, and the estimations are sufficiently accurate. High determination coefficients and low standard errors of estimate indicate that VO2max estimation by AOT protocols is considerably trustworthy.