The purpose of this study was to determine the effect of endurance exercise and a detraining period, on lipid peroxidation (MDA) and response of antioxidant system (FRAP, uric acid, bilirubin and total protein). For this reason 35 male rats divided randomly in two groups: Endurance group (experimental, n=20) and Control group (n=15, without any program training). Experimental group trained for 12 weeks, 3 times per week, with given intensities (five trained rats, experienced the detraining, so that the effects of detraining studies, from 8th to 12th week). During our experiments, animals were individually housed in a climate-controlled (temperature and humidity) laboratory animal, and were had 12:12 h light-dark cycle, and standard rat chow. Measurement of MDA and FRAP performed manually, while other measurement accomplished by kit.Data analyzing by using Two Way Repeated ANOVA showed: There were no significant difference between two groups about any of variables, but in the endurance group, there was significant difference through various assessment about uric acid (p=0.000) and bilirubin (p=0.000). Generally, this study showed that endurance exercise training don’t cause oxidative stress (lipid peroxidation), however some adaptation in antioxidant system established.