Armaghane Danesh
Year:2018 | Volume:23 | Issue:2 (127) |Papers Count:14

Background & Aim: Tissue engineering is a new method for the replacement of degraded tissue components by biodegradable polymers, which is provided as a three-dimensional scaffold for growth and proliferation of stem cells. In this study, chitosan scaffold was used to evaluate the proliferation of fibroblasts in the presence of hyaluronic acid. Methods: In this experimental study, powder scaffolds were prepared for growth of fibroblastic cells. The following groups were designed for later studies: Group 1: Chitosan scaffold with hyaluronic acid, Group 2: Chitosan without hyaluronic acid scaffold, Group 3 (control 1): Hyaluronic acid fibroblast cell and Group 4 (control 2): Hyaluronic acid fibroblaster cell. The human foreskin was prepared and the fibroblasts of the dermal layer were removed after separation, and the cells were transferred to the culture flasks with DMEM medium and stored in a CO2-containing incubator. After several passages, 10, 000 cells were transferred to 96 wells containing DMEM medium and MTT and DAPI staining method was used to amplify fibroblasts on the chitosan scaffold. The obtained results were analyzed by ANOVA and Tukey's post hoc test after uniformity of data analysis. Results: The mean survival rate of chitosan without hyaluronic acid scaffold in 24 hours was significantly higher than that of control with and without hyaluronic acid and chitosan group with hyaluronic acid (P <0. 05). The mean survival in the control group without hyaluronic acid increased significantly in 48 hours compared to the chitosan scaffold with and without hyaluronic acid and the control group with hyaluronic acid (P <0. 05). The mean survival time in chitosan scaffold with and without hyaluronic acid in 72 hours was not statistically significant compared to control groups with and without hyaluronic acid. Conclusion: Chitosan scaffold showed better biocompatibility with fibroblasts due to its hydrophilic property, but the presence of hyaluronic acid with chitosan reduced the fibroblast growth trend. Chitosan may be alone in structures that are synthesized to repair damaged areas of the skin, a good scaffold for proliferation of damaged fibroblast cells.

Background and Aim: Gentamicin is an antibiotic belong to a grouped of aminoglycoside used to treat a variety of bacterial infections particularly gram-negative ones. However, the nephrotoxicity and oxidative damage caused by gentamicin limit its long-term clinical use. Green tea contains some phenol compounds called catechins which have antioxidant and anti-inflammatory effects. AQP1 is proteins that make a water channel in renal tubules that transported water during the appical and baso-lateral of the renal tubules. The aim of this study was to evaluate the effect of green tea extract on expression of AQP1 gene in Gentamicininduced renal toxicity in male Wistar rats. Methods: In this experimental study, 30 male Wistar rats aged 4-6 weeks (Weighing 160-200 grams) were divided into 5 groups of six: control group (no treatment), sham group (intraperitoneal injection of physiologic serum), group Experimental group 1 (intraperitoneal injection of 20 mg / kg gentamicin), experimental group 2 (intraperitoneal injection of green tea with a dose of 200 mg / kg), experimental group 3 (intraperitoneal injection of gentamicin and 200 mg / kg of green tea). All trated was performed intraperitoneally for 10 days. Blood biochemical changes included creatinine and urea levels based on Bertolt, Chlorometric and Gaffa method were determiend. At the end of exprement the renal changes were evaluated by means of histochemistry and immunohistochemistry. Data were analyzed by one-way ANOVA. Results: The mean serum level of urea and creatinine in group 1 was significantly increased in comparison with the control group (P<0. 05) and the mean serum level of urea and creatinine in the third group was in comparison with the control group was significantly increased (P<0. 05). Renal tissue necrosis and inflammation, accumulation of eosinophilic hyaline substances in some renal tubules, infiltration of inflammatory menonocellular cells and reduction of expression of AQP1 gene in the proximal tubule caused by gentamicin with a dosage of 200 mg / kg of green tea extract were not improved. Conclusion: From the findings of this study, it can be concluded that green tea extract in this dose and duration can not have a protective effect against the renal toxicity of gentamicin.

Background & Aim: Fat-Specific Protein 27 (Protein Specialty Protein) is one of the proteins that play a role in the regulation and metabolism of lipid droplets. The aim of this study was to evaluate the effect of endurance training on expression of specific lipid profile (FSP27) and insulin resistance in STZ-diabetic rats. Methods: In this experimental study, 40 male Wistar rats were divided into five groups (diabetic group), low intensity endurance training (DLE), diabetic group, moderate intensity exercise (DME), diabetic group, and endurance training with intensity High (DHE), control group of diabetic (DC) and healthy control (HC). After diabetic administration, streptozotocin was injected with endurance training with low, moderate and high intensity for eight weeks, three sessions per week and each session for 30 minutes. Serum glucose levels were measured by glucometer, insulin with mouse specific ELISA kit, relative expression of FSP27 protein with Western Blot method and insulin resistance index. One-way analysis of variance and Tukey and James Howell post hoc tests were used to determine the difference between groups. Results: The results showed that endurance training with three severity (low, moderate and high) had a significant effect on serum glucose, insulin and insulin resistance values (p = 0. 001). Reductions in serum salivary and glucose levels were significant in high intensity exercise groups and moderate intensity exercises versus diabetic control and low intensity exercise (p≤ 0. 05). Insulin resistance values were significantly higher in moderate and high intensity training groups compared to low exercise group, diabetic control group and healthy control group (p≤ 0. 05). The expression of FSP27 protein in endurance training groups with three intensities with diabetic control and healthy control was not significantly different. However, the results showed that the content of FSP27 in the training groups increased with intensity of exercise compared to the diabetic control group (the value of p was exactly noche, not approximation) Conclusion: Although, none of the endurance training interventions produced a significant difference in FSP27. Considering the tendency to increase FSP27 with increasing endurance training, it seems that endurance training with an appropriate intensity can be achieved by increasing FSP27 and subsequent reduction of the acids Free fat on the one hand and increased glucose uptake, on the other hand, increase insulin sensitivity and improve insulin resistance in diabetic rats.

Background and Aim: Marital adjustment is one of the most important factors affecting family function. According to communication approaches, the existence and training of communication inhibitors has a significant role in increasing marital adjustment and reducing the problems of intimate relationships. The purpose of this study was to investigate the effect of practical application of intimate communication skills (PAIRS) on marital adjustment. Methods: This quasi-experimental study was a pretest-posttest design with control group. The statistical population consisted of women who were referred to an intervention center in individual, family and social crises in Bojnourd in 1395 due to incompatibility and marital problems. The sample consisted of 26 people who were selected by convenience sampling method and randomly assigned to experimental and control groups (each group was 13). The data collection tool was Esperner's marital adjustment questionnaire, completed before and after intervention by both groups. The experimental group participated in 8 sessions of two hours of practical training in communication skills and the control group did not receive any intervention. Data were analyzed using one-variable covariance analysis (ANCOVA) and multivariate analysis of covariance (MANCOVA). Results: The findings of this study showed that the practical application of communication skills is effective in increasing marital adjustment (p<0. 05). Also, the practical application of communication skills increased the marital satisfaction dimensions (p<0. 05), marital correlation (p <0. 05), marital satisfaction (p<0. 05) and marital affection (p<0. 05). Conclusion: Practicing practical use of communication skills increases marital adjustment. Therefore, in counseling and marital interventions, it is possible to use intimate communication skills to improve couples' relationships and increase their compatibility.

Background & Aim: Ginger possesses antioxidant properties which modifies oxidative indices. The use of high fat diet and low mobility is one of the reasons for the prevalence of fatty liver disease. Therefore, this study aimed to investigate the effect of ginger extract and physical activity on tissue changes induced by high fat diet and serum liver enzymes in adult male rats. Methods: In this experimental study, 56 adult male rats in control (non-treated), control (treatment with high fat diet (10 ml / kg) and 5 experimental groups receiving high-fat diet (10 ml / kg) plus Doses of 100 and 200 mg / kg of ginger extract alone and with physical activity on the treadmill were used. Prescriptions were taken 28 days and served as gavage. At the end, after anesthetizing the animals and collecting blood from their hearts, their liver was removed to measure the AST, ALT and ALP enzymes, and after preparation of the tissue sections, the results of liver enzymes were measured by Anova and Duncan tests and significant differences in the data at 0. 05 > p was considered. Results: High fat diet increased AST, ALT and ALP enzymes at the level of p <0. 001 and liver tissue damage in comparison to the control group, and ginger and physical activity alone or with each other significantly decreased the AST and ALT enzymes at p >0. 01 and ALP at the level of p <0. 001 and improve the liver tissue structure compared with the high-fat diet group. Conclusion: The results showed that high levels of ALT, AST, ALP, liver tissue damage, physical activity and ginger extract alone and in combination decrease ALT, AST and ALP and improve the liver tissue structure. It is better to modify the results based on the results, similar to the findings.

Background & Aim: According to the disturbance in testis circulation and subsequently the anemia of an organ, it can disrupt fertility. In this study, the protective effect of mesenchymal gastric lavage cells on testicular histomorphometry after the torsion of Tursion-Detursion (T-D) of the testicular band was investigated in a comparative manner. Methods: In this experimental study, 30 adult mice were randomly divided into three equal groups; the control group was Sham, in this group only the abdominal cavity was opened and then closed. Torsion Torsion Group (T-D), in this group, the fracture torsion was performed in the left testicular fracture. After the circulating blood was restored to the rat's ellipse, a phosphate buffer of 10 μ L was injected. Mesenchymal cell group (T-D + Msc), after torsion of the defect in mice, the mesenchymal cells were injected into half a million volumes, 10 microliters, of the testicular network. At the end of the treatment period (35 days), after sampling the testicular tissue of the left side and stabilizing the sample, paraffin sections were stained with Hematoxylin Eosin and Masonium trichrome (for cervical counts), then the tissue parameters were evaluated. The data were analyzed using ANOVA test and Tukey post hoc test. Results: Our results showed that mesenchymal treatment (T-D + MSc) had a significant increase in the mean spermatogenesis, active serotylated cells and leydig cells, diameter of the menisis tubes, epithelium thickness and testis capsule compared with the defect torsion group (TD) P<0. 05). In the study of the number of arteries in the T-D group, there was a significant decrease in the T-D + MSc group (p<0. 05). Conclusion: Using of Mesenchymal cells can improve the process of repairing the damage caused by torsion defect in the testicles by increasing angiogenesis and tissue repair.

Background and Aim: Diabetes is known as the most common metabolic endocrine disorder that causes debilitating complications of the eye, renal, neurological and cardiac diseases, and has irreparable biological, psychological, social and economic consequences. The aim of this study was to determine the effect of water exercise on glycosylated hemoglobin and C-reactive protein in type 2 diabetic women. Methods: In this semi-experimental study, 30 type II diabetic women were selected and randomly assigned to either control (n = 15) or control (n = 15). The experimental group performed 8 sessions per week for 3 sessions and 60-70 minutes each exercise protocol in the cramped pool. During this study, no intervention was performed on the control group. Data were analyzed for the comparison of intra-group of correlated T-test and inter-group comparison of independent t-test (p<0. 05). Results: The result showed that the exercise in water in the experimental group, body mass index (p <0. 015), waist to hip ratio (p <0. 022) and body fat percentage (p> 0. 04) significantly decreased. Also, the results of independent t test showed that HOMA-IR (P=0. 013), CRP (P=0. 001), HbA1C (P=0. 001), significantly decreased in experimental group compared with control group. Conclusion: It can be suggested that eight weeks of exercise in water by decreasing anthropometric indices, CRP levels, glycosylated hemoglobin, and insulin resistance index can prevent type 2 diabetes and its metabolic complications.

Background & Aim: Addiction to Substances has led to a decrease in productivity and inefficiency in psychological issues, including psychological well-being among drug users. The purpose of this study was to investigate the effectiveness of group cognitive hypnotherapy on psychological well-being in people referring to residential and semi-residential centers of addiction in Yasuj. Methods: This is a quasi-experimental study with pre-test, post-test and control group. The statistical population consisted of all drug dependent patients referring to residential and semi-residential of addiction centers in Yasuj city. 40 patients were selected by convenience sampling method and randomly assigned to experimental and control groups. The instrument used in this research was Rif's psychological well-being questionnaire. Group intervention of cognitive hypnotherapy was performed on the experimental group during 8 sessions of 90 minutes and once a week, but no intervention was taken on the control group. At the end of the intervention, both groups (experimental and control) were subjected to post-test. To analyze the collected data, covariance analysis was used. Results: The mean and standard deviation of the pre test of psychological well-being in the experimental group was 37. 87 ± 3. 42 and 39. 25 ± 2. 86 in the control group. Also, the mean and standard deviation of posttest psychological well-being score in the experimental group was 66. 45 ± 15. 61 and 39. 15 ± 2. 73 in the control group. The results showed that group cognitive hypnotherapy increased the psychological well-being of the experimental group in comparison with the control group at a significant level (p = 0. 001). Conclusion: Group cognitive hypnotherapy has been effective in improving psychological symptoms in patients with substance dependence in people who are referred to residential and semi-residential centers of addiction treatment. It has led to increased psychological well-being in drug-dependent patients.

Background & Aim: Brucellosis is a zoonotic infection caused by the bacterial genus Brucella. That is transmitted from animals to humans. The disease is considered as a major problem in many regions of the world, including Iran, which is a source of income and employment. The aim of this study was to investigate the epidemiological data of reported cases of brucellosis in children and adolescents in Kohgiluyeh and Boyerahmad province. Methods: In this cross-sectional descriptive-analytical study, all children and adolescents aged 1 to 18 years old who had been diagnosed with brucellosis during the years 2009-2015 and had their information in Kohgilouyeh and Boyerahmad Province Health Center entered the study. Data were analyzed using Chisquare and Mann-Whitney U. S. tests. Results: The total number of people with brucellosis was 81, of which 40 (49. 4%) were male and the rest (50. 6%) were female. 67 (82. 7%) of the patients were resident in the village and nomads and 14 others (17. 3%) were lived in the city. The mean age of the patients at the time of diagnosis was 11. 30 ± 4. 73 years and the most prevalent age group was 11-18 years with 61. 7%. The disease was more prevalent among the students (48. 1%) and in the first six months of the year (79%). The most common way of transmission of disease was consumption of suspected dairy products and simultaneous contact with the livestock (85. 2%) and (81. 5%) respectively. About 82. 8% of the patients had diagnosis between 0 and 3 months (acute brucellosis) and 17. 2% were amonge 3 months to 1 year (subacute brucellosis). The most common clinical signs of observation were muscle pain, bone pain with 74. 1%, fever with 58%, low back pain with 55. 6%, weakness and anorexia with 42%, weight loss of 32. 1%, weakness and anemia 2. 5% and depression was 1. 2%. There was a significant difference between the incidence and history of vaccination of livestock (p <0. 001). In wright test, 28. 4% of the patients had titers, and in the case of coombs wright and 2ME test, no cases were considered 4. 9% and 12. 3%, respectively. Conclusion: Regarding the prevalence of the disease in children and students of rural and nomadic areas, early detection and control of the disease in this group, which is one of the active groups of the community, is necessary. In addition to vaccination of livestock, educating this community about the lack of contact with infected animals and the non-use of non-pasteurized dairy can help.

Background & Aim: Therapeutic schemas model emphasizes the role of early maladaptive schemas in psychological disorders. The aim of this study was to determine the effectiveness of schema therapy on the severity of depression and suicidal thoughts in patients with depressive disorder. Methods: This research was a semi experimental study using pre-test and post-test with control group. among all patients with treatment resistance depression visited in Naft and Sadooghi hospital of Tehran, 30 patients were selected through inclusion-exclusion criteria and availability sampling method then randomly were assigned into two equal experimental (15 persons) and control (15 persons) groups. Experimental group experienced 12 weeks of schema therapy, while control group were just followed. Beck depression inventory (BDI-II) and Beck suicide scale ideation (BSSI) were used in pre-test and post-test as the. Data were analyzed by Mancova via SPSS software. Results: The results of this study showed that the mean scores of depression and suicidal ideation decreased significantly in the experimental group in the post test compared to the control group (p <0. 0001). In other words, schema therapy sessions reduced the severity of depression and suicidal thoughts in patients with depression resistant to treatment. Conclusion: The results indicate that schema therapy can be used as an effective intervention to reduce the severity of depression and suicidal thoughts in people with depressive disorder. Therefore, it is suggested that psychologists and psychiatrists use a therapeutic schema to reduce the psychological problems of people with depressive disorder.