Armaghane Danesh
Year:2018 | Volume:23 | Issue:4 (129) |Papers Count:11

Background & Aim: This study aimed to determine the pretreatment effect of 6-week resistance exercise with injections of vitamin D3 supplementation on the levels of klotho in female Lewis rats with experimental autoimmune encephalomyelitis. Methods: The present experimental study was conducted on 45 female Lewis rats with an average weight of 111. 3 ± 1. 60 grams which were randomly divided into 9 groups (n=5 per group), including, healthy control, EAE control, sham, Resistance training, EAE resistance training, vitamin D3, EAE vitamin D3, combination, EAE combination. The resistance-training program included climbing a ladder with tail-loaded weights. In addition, vitamin D3 groups received vitamin D3 supplementation (2ng/0. 2mg sesame oil/every two days) intraperitoneal for 2 weeks. To induce EAE model, to each animal in 4. 0 ml of solution guinea pig spinal cord and equally adjuvant and, was injected klotho levels were measured by ELISA. For data analysis, both one-way ANOVA and Tukey test were used with a significance level of p≤ 0/05. Results: The results of this study indicated that the mean serum levels of klotho in the brain tissue of control group were significantly different from control group (p=0/001). Klotho levels in the resistance training group were significantly higher than the control group (p=0/001). In addition, there was a significant increase in klotho in supplementation and combination patient group compared to the control group (Respectively P=0/001؛ P=0/006). Furthermore, the difference between the training, supplementation and combination groups was not significant (p≥ 0/05). Conclusion: The result of the present study revealed that pretreatment with the resistance training and injection of vitamin D3, alone and in combined together was able to prevent the reduction of klotho's levels against EAE induction. As a result, this protocol is likely to have a protective effect against MS.

Background and Aim: Visfatin is an insulin-like protein derived from adipose tissue that is associated with obesity and insulin resistance. The purpose of this study was to compare the effects of 12 weeks of combined exercises and vitamin D on the serum visfatin level and beta cell function in women with overweight type II diabetes. Methods: 40 type 2 diabetic women with a mean age of 1. 49 ± 3. 65 years, weight 158. 15 ± 5. 65 cm and body weight index 29. 4 ± 0. 46 kg / m2 were selected purposefully The groups were randomly divided into four groups of 10, three experimental groups (combined exercise, vitamin D, combined exercise with vitamin D) and control group. Height, weight, BMI and fat percentage of subjects were measured. After 12 hours of fasting, the primary blood sample was taken to measure glucose, insulin, visfatin, insulin resistance, and beta cell function. Subjects then participated in the experimental group 1 in the combined program. The experimental group, in addition to the combined exercises, consumed vitamin D 6 pills per 50, 000 units a week. Experimental group 3 consumed only vitamin D with 6 pills per dose of 50, 000 units per week and did not have any training. The control group was only tracked. After 12 weeks, all variables were re-measured in 4 groups. Data were analyzed using covariance analysis (ANCOVA) at the level of p <0. 05. Results: The mean values of post glucose, insulin, insulin resistance, beta cell and visfatin function were significantly different between the four groups with control of pre-test values of these variables (0. 001> p). In the case of insulin, there was no significant difference between the mean values of insulin levels in vitamin D, exercise and combination therapy and vitamin D (p <0. 05). There was no significant difference in insulin resistance between the mean resistance to insulin in the control and vitamin D groups (p> 0. 05). There was no significant difference in the performance of beta cells between the mean values of performance in control, exercise and exercise groups and vitamin D (p <0. 05). In the case of visfatin, the mean values of visfatin in the treatment group and vitamin D were significantly less than the other three groups (p <0. 05). Conclusion: The findings of this study indicate the effect of combined exercises and vitamin D intake on visfatin levels in overweight diabetic women.

Background & Aim: Students face a lot of emotional problems and stress that affect their individual and adaptation as well as their quality of life. One of the things that threatens their health is emotional failure. Logo therapy is one of the existing approaches in the therapy field, the efficiency of which has been established for resolving many emotional problems. The purpose of the present research was to investigate the efficacy of group logo therapy on Symptoms of emotional failure and Emotion Cognitive Regulation of female students with love trauma syndrome. Methods: The present research was of applied research type. The research method was semiempirical with a pretest-posttest with control group design. Populations were all female students of Rasht Azad University and were selected through voluntary sampling method. Sample numbers were 30 students that gained high scores on Love Trauma Inventory (1999) and had the lowest score in Emotion Cognitive Regulation (2006) randomly assigned to control and experiment groups (each group included 15 students). The experiment group exposed to 10 instruction sessions of group logo therapy. But the control group did not receive any interventions. The data were analyzed through descriptive statistics method and multivariate covariance analysis (MANCOVA) in SPSS v. 20. Results: The multivariate covariance analysis results for Symptoms of love Trauma (F=5. 69), Make it less important (F=181. 51), Reappraisal (F=232. 25), Reprogramming (F=366. 27), Focus again (F=399. 34), Acceptance (F=11. 95), Blame others (F=307. 25), Catastrophe (F=51. 53), Mental crawl (F=63. 11), and self Blame (F=51. 20), suggested that there was a significant difference between the Girls of experiment and control groups (P< 0. 01). According to the posttest scores of the experiment control groups for these two variables, it was recognized that the Group Logo therapy has led to reduction in Symptoms of love Trauma, reduction in Incompatible strategies for cognitive emotion regulation and increase in Compatible strategies for cognitive emotion regulation among the Girls of the experiment group in comparison to the control group in the posttest stage. Conclusion: The results of this study show that participating in Group Logo therapy meeting can affect Reduce on Symptoms of love Trauma and promotion of Emotion Cognitive Regulation of female students with love trauma syndrome. Therefore resistance to stress and Negative emotions can be increased by Life Meaningfulness and doing so promote health.

Background and Aim: evidence has shown the relation between autonomic nervous system response and sleep quality; and the beneficial effects of exercises on these two subjects. The present study was aimed to investigate the effect eight weeks selected swimming training on Autonomic Nervous System responses assessment and sleep quality changes in untrained females. Methods: In the present semi-experience study, 24 sedentary healthy untrained females were randomly assigned in two exercise training (n=12) and control (n=12) groups. The swimming training protocol includes sessions with 60 minutes at 60 to 80 percent of maximum heart rate, three sessions per week for eight weeks. Resting and maximal Heart rate assessed before and after intervention. Participants sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI). The data was processed by using SPSS version 22 and Independent and dependent t-test. Results: The results showed a significant decrease in resting heart rate and maximum in the exercise group (p = 0. 071, p = 0. 071, ) respectively. The score of sleep quality index of participants in the training group showed a significant decrease (p = 0. 0001). The performance of swimming exercises resulted in a significant decrease in the score of sleep quality index and maximum heart rate in the exercise group compared to the control group (p = 0. 002 and p = 0. 017). Quality and maximal Heart rate in training group as compared to control group (P=0. 002, P=0. 017). Conclusion: according to the findings, swimming exercises through improving the sympathovagal balance led to decrease Heart rate and improve sleep quality in untrained females. Thus it should be considered as a viable non-pharmacological strategy to improve sleep quality in untrained females.

Background and Aim: Overuse of salt is one of the most important risk factors for cardiovascular disease and is the first cause of mortality worldwide. The aim of the present study was to extract the views and opinions of the people of Yasuj about salt intake in order to use its findings in designing and implementing a behavioral intervention to reduce salt intake. Methods: In the present qualitative study, which was done by content analysis approach, the data were collected through six sessions of centralized discussion with presence of 66 residents of Yasuj, Iran. The data collecting tool was grouped in semi-structured questions. The comments presented were fully recorded, implemented and analyzed with the help of the content analysis method manually. Finally, five main themes and 31 sub-themes were identified and after several readings, the main themes were grouped together with similar meanings. Results: Five main themes were self-assessment of the level of salt consumption (with 5 subfields), side effects of too much salt (with three subfields), low barriers salt intake (11 subfields), strategies to reduce salt intake (8 subfields), communication channels were identified for communicating with individuals and encouraging them to reduce salt intake (with four sub-topics). Most participants in the study were aware of their salt intake, knew the side effects of too much salt in hypertension, educational programs at home and with minimal time and for all members of the family, and offer alternatives healthy for salt offered, Physicians and health care providers in the service centers considered the most influential people to modify their behavior. Conclusion: To improve risk behaviors such as high salt intake in the community, it is important to know their views and opinions so that effective audience-based interventions can be designed and implemented.

Background and Aim: Adiponectin is the most abundant peptide extracted from adipose tissue, which plays an important role in development of obesity. The purpose of this study was to evaluate the association of single-nucleotide polymorphism rs2241766 (+45T>G) with biochemical and anthropometric parameters in patients with non-alcoholic fatty liver. Methods: The present case-control study was performed on 80 patients with non-alcoholic fatty liver and 80 healthy peoples as control (77 men and 83 women). Triglyceride, total cholesterol, HDL, LDL levels and adiponectin levels were measured by standard methods using Pars Azmoon kit and ELISA kit respectively. Genotyping was also performed by PCR-RFLP method. Data were analyzed using independent t-test, Mann-Whitney, Chi-square and Kolmogorov-Smirnov tests. Results: Frequency of TG genotype was higher in patients (52. 4%) and frequency of TT genotype in healthy people (52. 7%), but regression analysis showed that rs2241766 polymorphism genotypes did not play a role in fatty liver disease (p<0. 05). The present study showed that none of the variables in the carriers of TT, TG, and GG genotypes was significantly different in the healthy group (p<0. 05), however, in the patients, the level of cholesterol in the genotypes was significantly different (p=0. 02). Conclusion: The results indicated that although the frequency of GT genotype and G allele in nonalcoholic fatty liver were more common. No significant relationship was seen between rs2241766 polymorphism in adiponectin genemm, non-alcoholic fatty liver disease and adiponectin levels.

Background and Aim: Leishmaniasis is one of the most common epidemic diseases in tropical regions of the world. The disease is affected by economic, social, cultural, environmental and climatic factors. Fars province is one of the areas with high prevalence of this disease. Due to the occurrence of climate change in recent years in the country and in this province, the present study was conducted to evaluate the epidemiology of cutaneous Leishmaniasis in Marvdasht city in 2017. Methods: This descriptive-analytic study was conducted on the epidemiology of cutaneous Leishmaniasis in Marvdasht city in 2015. Accordingly, demographic data of all patients registered in the Marijuana Medical Center's Marijuana Infection Unit were extracted and analyzed by descriptive statistics, independent t test, and Kruskal Wallis using SPSS version 16 software. Results: The incidence of disease in the whole population was 13. 44, in men 14. 4 and in 12. 4% in 10 thousand people. Most cases were in the age group of 21-30, housewives, and villagers. The hands had the most lesion and most people had more than 5 lesions. Also, the incidence of illness in the fall season was higher than in other seasons. There was no statistically significant difference in the incidence of disease based on gender (p <0. 05). Conclusion: Based on the results of this study, the incidence of cutaneous Leishmaniasis in the city of Marvdasht is high and there is no significant difference in gender-related illness. The incidence of illness was higher in housewives and residents of the city. As a result, appropriate health Proceedings, including controlling the vector and the reservoir of the disease, should be carried out by the authorities through the spraying and optimal treatment of patients, so that the spread and spread of the disease and the subsequent prevention of disease can be prevented.

Background and Aim: With respect to the increasing of antibiotic resistance in pathogenic bacteria, identification of new antimicrobial compounds is necessary. Bacterial pigments as bioactive products are one of the agents for identifying new antimicrobial agents. Microorganisms have been considered for the production of new microbial products such as pigments. Methods: In the present research, a total of 37 pigmented bacterial strains were obtained from soil samples of different regions of Fars province, Iran. These strains contained a wide variety of pigmentation including: red, yellow and green. Strains with different pigmentation were selected for further studies. Pigments were extracted by methanol-water or methanol for secreted and nonsecreted pigments, respectively. In order to evaluate the antibacterial activity of pigments, disc diffusion and macrodilution assay were used on MDR Klesiella isolated from clinical samples. Finally, microorganism with anti-bacterial pigment was identified by molecular method. Results: Based on the morphology of isolated pigment colonies in different environments and carrying out biochemical tests, the presence of bacteria such as, Pseudomonas aeroginosa, Serratia marcescens, Enterobacter sakazakii, Flavobacter mizutaii, Micrococcus luteus and Staphylococcus aureus were identified. The total frequency of Klebsiella isolates was 8. 5%. Of the 17 isolated isolates, 12 isolates were completely resistant to all antibiotics, and six isolates were sensitive to the imipenem antibiotic. All of Klebsiella isolates were multi drug resistance. A Red pigment from Serratia marcessence was the most potent and showed inhibitory effects on MDR Klebsiella. Conclusion: Isolated microorganisms from environmental resources contained a very diverse collection of pigments, where different pigment structures with different antimicrobial properties can be searched. The present study indicated that bacterial pigments can be effective against MDR Klebsiella isolates.

Background and Aim: Colorectal cancer (CRC) is the second most common cancer in developed countries. More than 10% of CRCs are inherited and include HNPCC and FAP ligation syndrome. The MSH2 gene is located on chromosome 2 (p21) and consists of 16 exons. MSH2 is a protein that plays a role in restorative MMR after DNA replication. The MSH2 protein is attached to MSH6 or MSH3 and forms MutSα and MutSβ complexes that identify small and large deletion / insertion abnormalities, respectively. The main objective of the present research was to evaluate HRMA's ability and effectiveness in detecting tri-nucleotide deletion mutations and determine the frequency of these mutations in homozygous and heterozygous states in colon cancer samples compared to control group. Methods: In the present study, a case control was carried out in 2016-2017 to detect the mutations of the three nucleotide deletions of GTG in the position of exon 12 of MSH2 gene. For this purpose, 50 samples of colorectal cancer with 50 healthy samples were studied. At first, genomic DNA was extracted from paraffin tissue samples and then examined by HRMA technique and direct sequencing of the triple nucleotide deletion mutant GTG. The controls used included a sequence of 96 bat games in wild type and 93 bp in the mutant mode. Equal portions of these two were used for the heterozygous state of this site. Finally, the number of samples in each group was compared in one way ANOVA and LSD mean comparison. Results: The number of heterozygous samples for the site in colon cancer samples was significantly higher than that of homozygous consecutive homozygos. In general, the results of the present study indicated that the HRMA technique had the ability to separate and remove the pairs of homozygos and heterozygotes according to their melting temperature (Tm) Conclusion: According to the results of this study, the frequency of removal of the three nucleotide GTGs in cancerous samples was significantly higher than healthy ones.

Background and Aim: Lupus is an autoimmune disease that can lead to the involvement of different organs. Its cause has not yet been fully understood, although the effects of factors such as environmental, genetic and hormonal factors have been identified. The purpose of this study was to report a case of lupus manifestation and bee sting. Methods: The present paper was a case-control study. Patient information was collected from clinical records and clinical records and were included in the questionnaire. The results of patient tests were also studied. Patient presentation: An 11-year-old boy was admitted to the hospital with the history of fever, malar rash and joint pain, with a 20 day suffering. The child had no presentations of the disease, until he was stung by a bee on his neck. Two weeks later, he developed generalized rash, malar rash, arthritis and photosensitivity. In lab data; anemia, proteinuria, increased levels of anti-nuclear antibody, anti-double strand DNA and erythrocyte sedimentation rate were observed. Class III of nephritis was observed based on the renal biopsy. Treatment with prednisolone and hydroxychloroquine was started and in one year of follow up, favorable outcomes were observed. Conclusions: Based on the presented case and the previous reports, it seemed that the bee venom might have had a dual role in the immune system. Although the beneficial effects of the bee venom had been observed in some autoimmune diseases (arthritis), some of the autoimmune diseases were triggered by the bee venom (such as Guillain-Barre syndrome, Henoch-Shonleine purpura, myocarditis and nephritis). The present study was of few studies reporting SLE following bee sting. Therefore, considering the reported adverse effects of bee venom, the application of bee venom in the treatment of diseases should be reconsidered.