Cold damage to flower bud is one of the limiting factors of almond growing in the world. This research was conducted as a factorial experiment in a completely randomized design with three replications to evaluate selected almond cultivars ‘A93’, ‘Bome’, ‘Rabie’, ‘Shahrod21’, ‘144 Yazd’and ‘Marcona’ responses to freezing damage based on laboratory tests. In this investigation, flower buds of almond cultivars in three phenological stages bud swell, popcorn, blossoming were subjected to different cold temperature. During experiment period some of physiological characters of bud flower (ion leakage, proline and soluble carbohydrate) and percentage of frost damage (amount of tissue browning) were evaluated. Statistical analysis showed that percentage of frost damage in almond is strongly related to phenological stage and genotype, so that in bud swelling stage at -10oC, frost damage percentages in almond cultivars ‘A93’, ‘Bome’, ‘Rabie’, ‘Shahrod21’, ‘144 Yazd’ and‘Marcona’ were 5.5, 85.17, 62.85, 56.4, 61.3 and 56.51% respectively. And also in popcorn stage percentage of cold damage in almond cultivars ‘A93’, ‘Bome’, ‘Rabie’, ‘Shahrod21’, ‘144 Yazd’ and‘Marcona’ was 54.4, 69.4, 76.1, 68.8, 70 and 75.5% respectively. In the blossoming stage at -3°C frost damage in almond cultivars ‘A93’, ‘Bome’, ‘Rabie’, ‘Shahrod21’, ‘144 Yazd’ and ‘Marcona’ was evaluated 65, 60, 70, 100, 100 and 100% respectively. Results of physiological characters study showed that in bud swelling stage ion leakage amount in almond cultivars and frost damage were directly correlated, but between frost damage and proline content at popcorn stage there was no significant correlation. Among the almond cultivars in the blossoming stage, soluble carbohydrate content was different, so that cultivars with high level of carbohydrate content had higher resistance to frost damage. In ‘Marcona’ and ‘Rabie’ cultivars with 24.2 mg g-1 fw and 23.3 mg g-1 fw percentage of frost damage was 60 and 100, respectively.