Anatomical Sciences Journal
Year:2011 | Volume:9 | Issue:36|Papers Count:10

Purpose: At the present time the role of stem cells and its therapeutic effects is well known. Human or animal (bone marrow or cord blood) stem cells in stem cell therapy are a promising attempt to improve the recovery after post embryonic injuries.Materials and Methods: The present study was carried out on young Wistar rats. At first, cord blood stem cells (CBSCs) were isolated, and labeled with Bromodeoxyuridine (BRDU). Animals were subjected to internal carotid artery occlusion (ICA) for 20 minutes. After that, 2x105 isolated CBSCs were injected into the tail vein of the experimental rats. The control animal group did not receive any injection and intact rats were assumed to act as control group.Results: The results were evaluated by the following parameters behavioral analysis in animals and neurological study, in damaged brain areas specially in frontal cortex region in experimental and negative control. It was shown that control animals did not return to their initial behavioral while the experimental groups (CBSCs injection), within 2 weeks after ICA, had parameters like intact rats. In addition, histological study reveals that, the size of the damaged region in the control group was larger in the experimental (CBSCs-treated) group. The positive control did not show any damage in the caudate nucleus or other region.Conclusion: It is concluded that the CBSCs transplantation is a beneficial influence upon the brain tissue reparation after hypoxic ischemic cell death in cerebral cortex area and frontal cortex region specially.

Purpose: At the present time the role of stem cells and its therapeutic effects is well known. Human or animal (bone marrow or cord blood) stem cells in stem cell therapy are a promising attempt to improve the recovery after post embryonic injuries.Materials and Methods: The present study was carried out on young Wistar rats. At first, cord blood stem cells (CBSCs) were isolated, and labeled with Bromodeoxyuridine (BRDU). Animals were subjected to internal carotid artery occlusion (lCA) for 20 minutes. After that, 2xl0 5 isolated CBSCs were injected into the tail vein of the experimental rats. The control animal group did not receive any injection and intact rats were assumed to act as control group.Results: The results were evaluated by the following parameters behavioral analysis in animals and neurological study, in damaged brain areas specially in frontal cortex region in experimental and negative control. It was shown that control animals did not return to their initial behavioral while the experimental groups (CBSCs injection), within 2 weeks after ICA, had parameters like intact rats. In addition, histological study reveals that, the size of the damaged region in the control group was larger in the experimental (CBSCs-treated) group. The positive control did not show any damage in the caudate nucleus or other region.Conclusion: It is concluded that the CBSCs transplantation is a beneficial influence upon the brain tissue reparation after hypoxic ischemic cell death in cerebral cortex area and frontal cortex region specially.

Purpose: Human umbilical cord matrix (UCM) (Wharton jelly) stem cells labeling are tracking by MRI Materials and Methods: After 48 hours incubation with USPIO human umbilical cord matrix (UCM) stem cells were labeled with USPIO by the means of receptor-mediated endocytosis. Prussian blue staining and Atomic absorption spectroscopy were performed to identify and show the iron oxide nanoparticles in Wharton jelly stem cells. USPIO-labeled cells were tracked by 1.5T MRI in vivo after implantation to rabbit brain.Results: The results showed: (1) umbilical cord matrix stem cells could be labeled with USPIO and labeling efficiency was 90%. Prussian blue staining showed numerous blue-stained iron particles of USPIO in the cytoplasm of these stem cells; (2) - T2 image parameters in 1.5 T MRI was 660 ms and 120 ms respectively, (4) Remarkable low signal area on T2WI could exist for nearly one weeks and then disappeared gradually in the transplanted area with labeled cells.Conclusion: It was concluded that umbilical cord matrix stem cells can be labeled effectively with USPIO, and can be traced by proper MRI imaging in a prescribed and limited period of time.

Purpose: The aim of present study is to evaluate the effect of a pulsed infrared low-level laser on wound healing in an experimental induced diabetic rat model.Materials and Methods: 18 adult rats were divided into 2 healthy and 1 diabetic groups. Diabete was induced by intraperitoneal administration of streptozotosin in diabetic group. Two full - thickness skin incisions were made on the dorsum of rats. The wounds were divided into laser treated and sham wounds (groups). Laser-treated wounds of healthy animals were submitted to a pulsed-infra red 890 nm laser with 80 Hz frequency and 0.2 J/cm2 (200 s) energy densities for each point of wounds of the first and second experimental groups respectively. Laser-treated wounds of diabetic animals were submitted to the same laser with 0.2 J/cm2 (200 s) energy densities for each point of laser-treated wounds. On day 15 a sample of each wound were extracted and were submitted into tensiometerical test. Maximal force (MF, N) of samples was calculated.Results: Laser irradiation with 0.2 J/cm2 significantly increased maximum force of healthy (p=0.021) and diabetic (p=0.000) laser-treated wounds. Moreover p values of statistical analysis of laser-treated wounds of diabetic rats were better than those of healthy rats.Conclusion: Laser treatments with a pulsed infrared laser with 0.2 J/cm2 energy densities significantly accelerated wound healing in both healthy and diabetic rats. However, its positive effect , vas significantly better in diabetic rats.

Purpose: Nicotine can induce sub fertility or infertility both in males and females. The aim of this study is to evaluate the proliferative activity of adult mouse male germ cells following treatment with different doses of nicotine.Materials and Methods: Adult male NMRI mice were divided into four groups. The control group received normal saline and the other animals in groups of 2, 3 and 4 were treated with nicotine in doses of 0.1, 0.2 and 0.4 mg/Kg, respectively.Nicotine was administered intraperitoneally once a day for 14 days. All animals were dissected on 15th day, and evaluations were made by Ki67 immunohistochemistry staining and Johnson’s scoring for assessment of proliferative activity of mitotic & meiotic spermatogenic cells and evaluation of quality of spermatogenesis respectively, and Elisa test for serum testosterone level.Statistical analyses were performed using ANOVA and Tukey tests.Results: Nicotine in doses of 0.2 mg/Kg and 0.4 mg/Kg significantly reduced Johnson's score in comparison with that of control group (p<0.05).Nicotine in dose of 0.2 and 0.4 mg/Kg significantly reduced ki67 index in meiotic leptotene and pachytene cells in comparison with that of control group.However it was not effective on spermatogonia cells. Serum levels of testosterone in groups of 3 and 4 were significantly reduced compared to that of control group (p<0.05).Conclusion: This study indicates that nicotine has adverse effects on spermatogenesis process and it acts in a dose dependent manner. However, in lower doses, proliferative activity is not altered; therefore, it seems that probablility of the other mechanisms are involved in reducing of maturity and quality of spermatogenesis.

Purpose. The aim of this survey was to evaluate the effects of fatty acids, nutrients and vibration on bone mechanical properties and its metabolic parametr’s in rat.Materials and Methods: 56 male wistar rats weighting 150±30 were used. Rats divided into control and six experimental groups as below: Vibration, Vibration+nutrients, Vibration+nutrients+ Canola oil, Sunflower oil, Canola oil+Sunflower oil and Coconut oil. Experimental groups were treated for eight weeks with normal diet, vibration, 210 mg of Calcium, 55 IU of Vit D, 1mg of Boron and Oils amounting to %5 Of the normal diet of oils. After experimental period rats were killed and blood samples were collected, then femur and fifth lumbar vertebral bones excised.Mechanical properties of the lumbar vertebral bones and femoral neck were determined with the Axial Compression Test and Contilever Bending Test. Plasma samples were analyzed and metabolic parametrs levels were deterrnined. The data were analysed by one way ANOV A.Resalts: Mechanical properties of bones increased in the vibration group compared to those of control group. Nutrients caused significant decrease in mechanical properties of femoral neck compared to those of control group and vibration groups. Femoral neck stiffness and maximal load and Stiffness, ma-ximal load, energy to maximal load and deformation of the lumbar vertebral increased in the vibration+coconut group compared to the other groups.Conclusion: The findings showed that combination of vibration and coconut oil has beneficial effects on bone mechaninichal properties as well as metabolic parametrs especially on sex steroid hormonens.

Purpose: The purpose of this study is to present a cranifacial growth model using anthropometry based on changes observed in the human face.Materials and Methods: This cross sectional analytical study was conducted randomly on 45 pepole, with normal face patterns.Facial and cranial ratios were measured and compared. Data was analyzed by SPSS software. The regression line and the growth coefficient were determined for each Parameter. Finally, the mean values of these parameters were determined. Facial growth. pattern was drawn on the basis of on the growth parameters. A anthropometric ratios were obtained on the basis of facial landmark.Results: This research presents cranifacial growth model for young faces (2-18-years-old) using anthropometry based on changes observed in the human face that can be used as a method for growth parameters calculation.This model can also predict tailored face changes with the lapse of time.Conclusion: This model can be used for face recognition as well, on the other hand the results of this research can be used in medical professions such as maxillofacial surgery, growth and development studies, plastic surgery, bioengineering and non-medical fields such as shoe-making and eye glasses industries.