Anatomical Sciences Journal
Year:2006 | Volume:3 | Issue:4|Papers Count:9

Purpose: The purpose of this study was to determine acid phosphatase (ACP) activity of uterus in natural and pseudo pregnanat mice after ovulation stimulation usmg PMSG and hCG during preimplantation period.Materials and Methods: In this experimental research, 6-10 week old female NMRI mice were selected and divided into two groups: control and hyperstimulated. These mice were rendered normal pregnancy or pseudopregnancy using vaginal stimulatin. Five mice per each group were sacrificed by cervical dislocation at the first day to sixth day of pregnancy. For biochemical assay, samples obtained from the uterine horns were hemogenated , and then centrifuged with 14000g. The activity of enzyme was determined using paranitrophenyl phosphate as substrate, then specific activity of enzyme (V/mg) was calculalated according to the total protein (mg/dl). For hisotchemistry another sampels were obtained from uterus, then the cryosections were provided with 5 mm thickness and stained with Goumorys method.Results: The result showed that the biochemical findings were correlated with the histochemical observation. The ACP activity of uterus was increased from day I to day 4 of pregnancy and after that it was decreased. Almost there was no significant difference between ACP activity of hyperstimulated and non-stimulated groups.The enzyme reaction changes were seen mainly in epithelial cells of endometrium but their activity was constant in lamina propria and myometrium.Conclusion: Thus our results showed that increase in ACP activity in day 3-4 of pregnancy, emphasises the role of ACP in implantation. Also ovulation induction, can not change the pattern of ACP activity of endometrium during early pregnancy which requires further research.

Purpose: The objective of this study was to evaluate the relationship between some events that simultaneously occur during spermiogenesis, related to protamine deficiency,and acrosomal integrity with fertilization rate post ICSI.Materials and Methods: Semen samples from 68 infertile couples undergoing ICSI at Isfahan Fertility and Infertility center were assessed during this study. Semen analysis was carried out according to WHO criteria. Protamine deficiency and acrosin activity were assessed by Chromomycin A3 (CMA3) staining, and gelatinolyisis test. The fertilization rate in two CMA3 groups showed +/- 40 distinctive difference using t-test. Moreover, in this survey, mean of halo dimension and percentage of positive CMA3 sperms showed =/- 25 distinctive difference (deviation from the mean) with the fertilization rate.Results: Percentage CMA3 positivity and mean halo diameter show a significant correlation with fertilization rate. Fertilization rate was significantly different when patients were grouped for CMA3 positivity of 40%.Indeed mean on halo and CMA3 positivity were significantly different when patients were grouped for percentage of fertilization rate of 25%.Conclusion: Protamine deficiency appears to have a more significant effect on fertilization compared to the other variables evaluated.

Purpose: The exact role of nitric oxide (NO) in the wound has not been elucidated and the available reports are controversial. We tried to study the effects of NO on healing promotion of burns wound in rats. Materials and methods: 60 adult male rats weighing 250-270 gr. were used After induction of general anesthesia egion was exposed to 95c water for 8S in order to producing the wet burns. Then the animals divided into two categoriestegory have three proups: control, Expl which received 100mg/kg L-arginin intraperitonealy and Exp2 which received 10mg/kg L-NAME. Injections in the first category performed on the first, third and fifth days. Skin sampling was on seventh day after burn. Injections in the second category performed from the first to 13th day, every other day, and sampling was done on the 15th day. H&E, new epiderm and derm formation, the number of the blood vessels sections and sebaseous gland and neutrophil were counted in 10 areas of wounded increment by means of eye piece. Results: The rate of epiderm and derm formation the number of blood vessels and sebaseous gland in L- arginin groups were significantly higher than other groups. The number of neutrophils in L-NAME groups was significantly higher than other groups. Conclusion: The results showed that NO is effective in wound healing and with its induction during burn, probably it accelerates healing and prevents infection.

Purpose: This investigation was undertaken on 800 young women (18-25 years old) to determine the head and face phenotypes among them in two aborigines of Sistani (Fars) and Baluchi who were residing in southeats of Iran.Materials and methods: The current cross-sectional descriptive analytical study was performed on 800 normal young women 18-25 years old in two aborigines of Sistani (Fars) and Baluchi. Themorphological dimensions of head and face were measured with a scaled cephalometer and a sliding caliper Using the classical cephalometry .ephalic and prosopic indices were determined. The Chi-square and t-test were used to compare data. Results: Means and standard deviation of cephalic indices were 78.4±4.15 and 81.94±4.99 and prosopic indices were 83.22±4.02 and 84.86±5.15 in Sistani and Baluchi subjects respectively. Based on the cephalic index, the dominant and rare head types in Sistani group was mesocephalic (4 1.3%) and hyperbrachycephalic (6%), and in BIachui group was brachicephalic (42%) and dolichochephalic(5.5%) respectively. Furthermore, according to the prosopic index, the dominant and rare face type among sistanis were euryprosopic type (50.8%) and hyperleptoprosopic (0.5%) and in Baluchi group were euryprosopic (37%) and hyperleptoprosopic (2.5%) respectively. Conclusion: This research showed that differences in terms of head and face type indices between these two groups were statistically significant. Based on this cross-sectional study, it seems that there is differences between the aforementioned groups.

Purpose: The aim of this study was to see the effect of topical application of sodium phenytoin 1 % ointment and vaselin on open skin wound healing of male rat during inflammation, proliferation and remodeling phases.Materials and Methods: Experiments were carried out on 60 male wistar rats weighing 22S±25 gr. Rats were anaesthetized with ketamine, a 2cm circular wound in diameter was performed on the back of neck. Rats were divided into three groups: Experimental group - receiving sodium phenytoin 1 % on the wound every other day; Vaselin group - the wounds were dressed by vaselin; control group - no dressing was performed on the 3rd, 7th, 14th, and 28th day.Smples were prepared for routine histological study and were stained by hematoxylin - eosin, vangison and toluidine blue 1%. Fibroblasts, mast cells, and blood vessels were counted; depth of granulated tissue was measured and inflammatory cells and the chroma of staine collagen fibers were classified. Data were analysed by one and two way Anova tests.Results: Number of fibroblasts have been significantly increased in experimental group compare to control on day 3, 7, 14 (P<0.05). Number of mast cells have been significicantly decreased in experimental group compare to control on day 7 (P<0.05). Blood vessels were increased in phenytoin group compare to control and vaselin groups on day 3, 7, 14. The depth of granulation tissue was just increased on day 7 of experiment in phenytoin group. The number of inflammatory cells was decreased on the 7th, 14th day in phenytoin group. The collagen synthesis was started earlier in phenytoin treated group compared to the other groups. Conclusion: It seems that the application of 1 % sodium phenytoin not only reduces the inflammation and number of the mast cells, but also induces angiogenesis, fibroblast, collagen synthesis, and granulation of the injured tissue increment.

Purpose: This study designed to introduce Alizarin red S staining instead of Hematoxylin and Eosin staining in diagnosis of calcification of the paraffin sections. Materials and Methods: Uterine paraffin sections belong to the 20 women aged 53±4, endured hysterectomy stained with H&E and ARS staining. For H&E, the sections were treated with Mayer's hematoxylin (pH 2.3) for 5 min and with Eosin alcohol (pH 4.1) for I min respectively. For ARS, 1% ARS dissolved in distilled water was adjusted to pH 6.4. Aniline blue was used for background. Four sections were randomly selected for Microscopic study and vascular with medical calcification counted for each sections. Results: Thin crystals were seen clearly in most specimens stained with ARS, but were markedly reduced in tissue sections stained with H&E. The number of arteries with calcification (18.4±1.34 mean ± SD) was significantly (p<0.000) higher compared with H&E (10.4±1.42 mean ± SD). Conclusion: Standard H&E staining reduced the number of visible crystals, probably due to strong acidity of both Hematoxylin and Eosin solutions, where as the ARS stain seems to preserve a large number of the crystals, so we recommended this staining for a correct diagnosis of early stages of vascular calcifications.

Purpose: The aim of the present study was to identify the quantitative and qualitative changes of bronchiole and lung alveoli in Rat after exposure to welding fumes. Materials and Methods: A total number of 60 male Sprague Dawley Rats were divided into two groups: imental (40) and control (20). Rats of each group were subdivided into 2, 4, 6 and 8 subgroups. Experimental Rats were exposed to welding fumes for 2 hour/day and 5day/week in exposure chamber. According to timetable lung samples were taken from all groups and processed routinely, sections from paraffin blocks were stained by H-E, PAS, Van Gaisson, Alcian Blue pH=2.5 and PNA & WGA lectins. Epithelial thickness of bronchiole was measured after calibration of microscope and data were analyzed by Kruskall Wallis test. The amount of gases and particles measured in exposure chamber. Results: The results of the study showed the disorganized architecture and polarity of epithelial cells with nuclear hypercromasia in bronchioles and alveoli. The nature of secretary products of goblet cells was changed in experimental group. The increase in the amount of collagen fiber showed the fibrotic changes of lung. Kruskall Wallis test showed significant changes for epithelial thickness between control and experimental groups in 4 and 8 weeks (p<0.02). Conclusion: It seems that the bronchiole and alveoli responses to welding fumes follow an adaptive and reactive process. The severity of these responses is well correlated with the duration of exposure to welding fumes.

Inferior gluteal artery is the greatest branch of interior trunk of internal iliac artery that supplies the gluteal region and posterior of thigh. This artery emerges from inferior border of piriformis and descends toward the thigh deep to the gluteus maximus muscle. Several variations have been reported about inferior gluteal artery. In this case, the artery has emerged from superior border of pirifomis muscle and entered the gluteal region.