In arid climates, water is a limited resource, and turfgrass could be irrigated with wastewater. However, effect of irrigation with wastewater on nutrients and heavy metals uptake by plants needs to be evaluated. The objective of this research was to evaluate the effects of different percentages of advanced treated wastewater (TW) of Shahinshahr Wastewater Treatment Plant (100% water (control) with no TW, 50% water and 50% TW, and 100% TW with no water) on chemical elements uptake by four varieties of Zoysia grass (two varieties of Zoysia matrella and two varieties of Zoysiajaponica) in a loamy soil for a period of 10 months. A factorial experiment was carried out using a complete randomized block design with three replications. The results indicated that using TW for irrigating turfgrass increased plant uptake of Ni and Co. Maximum N, P, K, Fe, and Pb was measured in 50% TW irrigated soils. There was no significant variation in Pb uptake in these sources of irrigation water (drinking water of Isfahan and TW of Shahinshahr Wastewater Treatment Plant). Variation of K content was not significant. But varieties of Zoysia matrella (DALZM1 and DALZM2) absorbed more Ni and K than varieties of Zoysiajaponica (DALZJ1 and Mayko). Varieties of Zoysia japonica absorbed more Fe, Pb and Zn than varieties of Zoysia matrella. DALZM2 and DALZJ1 varieties absorbed the highest amount of Co and Ni. Interaction of percentages of TW and turfgrass variety indicated how the content of N, P, K, Fe, Zn, Co, Ni and Pb changed in different percentages of TW. The relation between elements uptake and increasing the percentage of wastewater in irrigation water was not linear.