Manesht-Ghalarang protected area in Ilam, Iran covers mountains with forest and is one of the most important natural habitats of Crown imperial (Fritillaria imperialis) of Liliaceae Family. This valuable and rare plant species exist in some locations in this area. This research introduces a new methodology for an ecological assessment and to understand the living environment ofF.imperialis and recommends new sites for rehabitating this plant. If natural or anthropogenic destructive factors such as fire, illness and pest occur in landscape, alternative habitats help the species remain in floristic list of area. The rehabitation was performed based on the plants existing environment. Conditions such as topography (elevation, slope and geographic aspect), climatic factors (rainfall and temperature), geology, land use, soil and companion vegetation were identified, mapped and overlaid using Geographic Information System (GIS). In order to achieve better accuracy in selecting new rehabitated sites, 30 samples of the ecological conditions ofF. imperialis eg. Soil quality parameters including Texture, acidity (pH), Electrical Conductivity (EC), Organic Material (OM) and nutrients (K, P, N, C, Ca, Mg and Na), were identified. In this study, The Ellen berg table was also used for the site evaluation. The results showed that 7 sites measuring 1.97 % (590 ha) of the 30, 000 ha total watershed area, is suitable for rehabitation of Crown imperial.