IN VITRO SOMATIC PROPAGATION OF ZIZIPHUS SPINA CHRISTTI (L.) WILLD VIA INDIRECT REGENERATION FROM LEAF EXPLANTS

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AHMADI E.; HOSSEINI NASR S.M.; JALILVAND H.; SALEHIAN AGHBLAQ H.

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Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2012 | Volume:20 | Issue:1 (39) Page(s): 111-123

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Title

IN VITRO SOMATIC PROPAGATION OF ZIZIPHUS SPINA CHRISTTI (L.) WILLD VIA INDIRECT REGENERATION FROM LEAF EXPLANTS

Author(s)

AHMADI E. | HOSSEINI NASR S.M. | JALILVAND H. | SALEHIAN AGHBLAQ H. | Issue Writer Certificate

Keywords

TISSUE CULTUREQ2

REGENERATIONQ2

CYTOKININQ2

AUXINEQ2

CALLUSQ2

ZIZIPHUS SPINA CHRISTIIQ2

Abstract

Regarding cross-pollination and a wide range of genetic variation in Ziziphus spina christti (L.) populations, vegetative propagation through TISSUE CULTURE techniques is necessary to reproduce the elite single plants. In this study effects of different levels of growth regulators on callogenesis and REGENERATION capabilities of CALLUS of leaf explants of the species were evaluated. Leaf explants of aseptic seedlings were cultured on MS medium containing 2, 4-D, NAA, TDZ and BAP levels (0.5, 1 and 2 mg/l). MS medium with different levels of BAP, TDZ and Kin (1, 3 and 5 mg / liter) was used for REGENERATION. Rooting process was done on 1/2MS medium with different levels of IBA and NAA levels (0.5, 1 and 2 mg /l). Results showed that the medium containing 2, 4-D and TDZ at concentrations of 0.5 and 1 mg/l (x=10) had the highest rate of CALLUS induction. Kin treatments (5 mg/l) and TDZ (1 mg/l) with averages of 8.84 and 8.95 had the highest rate of shoot production, but the shoot produced by TDZ treatments had an unusual shape, whereas, Kin treatments, produced normal shoots. Average rooting on IBA was higher than NAA treatments on all concentrations. One mg/l IBA showed the highest rate of rooting capability compared to the other treatments.

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APA: Copy

AHMADI, E., HOSSEINI NASR, S.M., JALILVAND, H., & SALEHIAN AGHBLAQ, H.. (2012). IN VITRO SOMATIC PROPAGATION OF ZIZIPHUS SPINA CHRISTTI (L.) WILLD VIA INDIRECT REGENERATION FROM LEAF EXPLANTS. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 20(1 (39)), 111-123. SID. https://sid.ir/paper/104479/en

Vancouver: Copy

AHMADI E., HOSSEINI NASR S.M., JALILVAND H., SALEHIAN AGHBLAQ H.. IN VITRO SOMATIC PROPAGATION OF ZIZIPHUS SPINA CHRISTTI (L.) WILLD VIA INDIRECT REGENERATION FROM LEAF EXPLANTS. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2012;20(1 (39)):111-123. Available from: https://sid.ir/paper/104479/en

IEEE: Copy

E. AHMADI, S.M. HOSSEINI NASR, H. JALILVAND, and H. SALEHIAN AGHBLAQ, “IN VITRO SOMATIC PROPAGATION OF ZIZIPHUS SPINA CHRISTTI (L.) WILLD VIA INDIRECT REGENERATION FROM LEAF EXPLANTS,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 20, no. 1 (39), pp. 111–123, 2012, [Online]. Available: https://sid.ir/paper/104479/en

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