Extraction of silk fibroin protein from Bombyx Mori cocoon by an optimized solvent system

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Ghanbari Hassan Kiadeh Saeed; RAHAIEE SOMAYEH

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL CONFERENCE ON MODERN TECHNOLOGY IN SCIENCES
Year:2023 | Issue:3

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Title

Extraction of silk fibroin protein from Bombyx Mori cocoon by an optimized solvent system

Author(s)

Ghanbari Hassan Kiadeh Saeed | RAHAIEE SOMAYEH

Keywords

Silk fibroin protein

Bombyx mori

Optimized solvent

Solubilization

SDS-PAGE

Abstract

Silk fibroin protein (SFP) has become an attractive option for biomedical applications due to its low cost, controllable biodegradability facilitates, unique mechanical properties, and high abundance. For all these applications,it needs to be extracted from its sources. In this study, we used an Optimized solvent system to extract SFP from Bombyx mori silk cocoons, and then the optical properties and quality of the extracted protein were evaluated by UV-Vis and SDS-PAGE analysis. Based on the results obtained, the presence of a maximum absorption peak in the range of 273 nm confirmed the presence of SFP. Also, by analyzing the prominent bands formed on the SDS-PAGE polyacrylamide gel and comparing it with the protein ladder, the presence of SFP in the solution was confirmed. These results can indicate the success of the method used in the Solubilization and regeneration of SFP in the present study.

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APA: Copy

Ghanbari Hassan Kiadeh, Saeed, & RAHAIEE, SOMAYEH. (2023). Extraction of silk fibroin protein from Bombyx Mori cocoon by an optimized solvent system. INTERNATIONAL CONFERENCE ON MODERN TECHNOLOGY IN SCIENCES. SID. https://sid.ir/paper/1050143/en

Vancouver: Copy

Ghanbari Hassan Kiadeh Saeed, RAHAIEE SOMAYEH. Extraction of silk fibroin protein from Bombyx Mori cocoon by an optimized solvent system. 2023. Available from: https://sid.ir/paper/1050143/en

IEEE: Copy

Saeed Ghanbari Hassan Kiadeh, and SOMAYEH RAHAIEE, “Extraction of silk fibroin protein from Bombyx Mori cocoon by an optimized solvent system,” presented at the INTERNATIONAL CONFERENCE ON MODERN TECHNOLOGY IN SCIENCES. 2023, [Online]. Available: https://sid.ir/paper/1050143/en

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