Purification and investigation of the antimicrobial effect of Lactobacillus plantrum toxin against Acinetobacter baumannii bacteria

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Khalaj Ayda; Mohammadzadeh Hosseini Moghri Seyed Amir Hossein; RANJBAR MOJTABA

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Seminar Paper

Paper Information

Seminar: INTERNATIONAL CONFERENCE ON MODERN TECHNOLOGY IN SCIENCES
Year:2023 | Issue:3

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Title

Purification and investigation of the antimicrobial effect of Lactobacillus plantrum toxin against Acinetobacter baumannii bacteria

Author(s)

Khalaj Ayda | Mohammadzadeh Hosseini Moghri Seyed Amir Hossein | RANJBAR MOJTABA

Keywords

Lactobacillus plantrum

Acinetobacter baumannii

antibiotic

bacteriocin

Abstract

Today, Acinetobacter, which is an opportunistic pathogen and is considered one of the most important causes of hospital infections, can cause a wide range of infections such as pneumonia and septicemia, and are resistant to most classes of antibiotics. Recently, the use of secreted substances of probiotic bacteria as an inhibitor and antibacterial has been noticed. This study was aimed at determining the antimicrobial activity of Lactobacillus plantarum against Acinetobacter baumannii common in hospital infections. After collecting and cultivating the bacterial strains, the toxin was purified from Lactobacillus plantrum by ammonium sulfate salt and the antibacterial effect of the toxin was evaluated after vertical electrophoresis (SDS-PAGE). The result of vertical gel electrophoresis showed a single protein band with a molecular weight of 25 KD indicates the correctness of the purification process of Lactobacillus plantrum toxin and the absence of any impurities after purification. Finally, the result of the antibacterial activity of the toxin showed the greatest effect at a concentration of 10 mg/ml and the least effect at a concentration of 2. 5 mg/ml on Acinetobacter baumannii bacteria, as a result of this antibacterial property is concentration dependent. Therefore, according to the promising results, this toxin can be an alternative strategy against the infection caused by Acinetobacter baumannii bacteria.

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APA: Copy

Khalaj, Ayda, Mohammadzadeh Hosseini Moghri, Seyed Amir Hossein, & RANJBAR, MOJTABA. (2023). Purification and investigation of the antimicrobial effect of Lactobacillus plantrum toxin against Acinetobacter baumannii bacteria. INTERNATIONAL CONFERENCE ON MODERN TECHNOLOGY IN SCIENCES. SID. https://sid.ir/paper/1050148/en

Vancouver: Copy

Khalaj Ayda, Mohammadzadeh Hosseini Moghri Seyed Amir Hossein, RANJBAR MOJTABA. Purification and investigation of the antimicrobial effect of Lactobacillus plantrum toxin against Acinetobacter baumannii bacteria. 2023. Available from: https://sid.ir/paper/1050148/en

IEEE: Copy

Ayda Khalaj, Seyed Amir Hossein Mohammadzadeh Hosseini Moghri, and MOJTABA RANJBAR, “Purification and investigation of the antimicrobial effect of Lactobacillus plantrum toxin against Acinetobacter baumannii bacteria,” presented at the INTERNATIONAL CONFERENCE ON MODERN TECHNOLOGY IN SCIENCES. 2023, [Online]. Available: https://sid.ir/paper/1050148/en

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