Standardizing 1,4-naphthoquinone measurement in Eleutherine Palmifolia: An hptlc-densitometry approach

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Mutiah Roihatul; Roziqin Ahmad Ainur; Fitrianingsih Avin Ainur; Rachmawati Ermin; Taufik Imam; Annisa Rahmi

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Journal Paper

Paper Information

Journal: JOURNAL OF MEDICINAL AND PHARMACEUTICAL CHEMISTRY RESEARCH Year:2024 | Volume:6 | Issue:5 Page(s): 481-491

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Title

Standardizing 1,4-naphthoquinone measurement in Eleutherine Palmifolia: An hptlc-densitometry approach

Author(s)

Keywords

linearity

Method Validation

4-naphthoquinone

HPTLC-Densitometry

Abstract

This study aimed to develop an HPTLC-Densitometry method for identifying and determining the 1,4-naphthoquinone compound in ethanol and water extracts of Eleutherine palmifolia (EP). The method was validated for linearity, accuracy, precision, Limit of Detection (LOD), and Limit of Quantification (LOQ). The linearity is assessed to ensure a linear relationship between the instrument response and various analytical concentrations. Accuracy is measured by comparing the measurement results with reference values or true values. Precision is evaluated through repeated measurements on samples with the same concentration, both within (intra-assay) and between assays (inter-assay). The Limit of Detection (LOD) determines the lowest concentration that can be detected, while the Limit of Quantification (LOQ) determines the lowest concentration that can be measured and quantified accurately with a specific level of confidence. The linearity test yielded an r-value of 0.9976, with an LOD value of 163.6006 ppm and a LOQ value of 495.7595 ppm. The method's accuracy ranged from 80-120%, and the precision value from %RSD was 0.99%. The 1,4-naphthoquinone content in EP ethanol extract was found to be 0.318% (w/w), while in water extract it was 0.092% (w/w). The method meets standard validation requirements and is recommended for use in traditional medicine development due to its high concentration of 1,4-naphthoquinone.

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