INVOLVEMENT OF ESSENTIAL LYSINE RESIDUES IN THE CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYROGENASE PURIFIED FROM STREPTOMYCES AUREOF ACIENS

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HAGHIGHI B.; FALAHATI F.

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Journal: IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE Year:2007 | Volume:31 | Issue:A3 Page(s): 313-320

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Title

INVOLVEMENT OF ESSENTIAL LYSINE RESIDUES IN THE CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYROGENASE PURIFIED FROM STREPTOMYCES AUREOF ACIENS

Author(s)

HAGHIGHI B. | FALAHATI F. | Issue Writer Certificate

Keywords

GLUCOSE 6-PHOSPHATE DEHYDROGENASEQ3

PYRIDOXAL 5’-PHOSPHATEQ3

ESSENTIAL LYSINE RESIDUEQ3

Abstract

Glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified and inactivated by pyridoxal 5′-phosphate (PLP). The inactivation was a pseudo-first order and time-dependent reaction. Complete inactivation was achieved at 0.2mM PLP within 16 minutes. The type of inhibition was competitive with respect to Glucose 6- phosphate. Spectral characteristics of PLP-enzyme complex corresponded to the formation of a Schiff’s base between PLP and lysine residue(s) of the enzyme. Intrinsic protein fluorescence sharply decreased upon PLP modification with about a 10 nm red shift. The presence of glucose 6-phosphate in the incubation mixture prevented the fluorescence change. Fluorescence studies revealed that NAD+ and NADP+ binding induces different conformational changes in pyridoxylated enzyme. The stochiometry of PLP binding to the enzyme showed that 2 moles of lysine residues were modified per mole of enzyme. The data indicated that the modified lysine residues are involved in substrate binding and/or catalytic activity of this enzyme.

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APA: Copy

HAGHIGHI, B., & FALAHATI, F.. (2007). INVOLVEMENT OF ESSENTIAL LYSINE RESIDUES IN THE CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYROGENASE PURIFIED FROM STREPTOMYCES AUREOF ACIENS. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, 31(A3), 313-320. SID. https://sid.ir/paper/117910/en

Vancouver: Copy

HAGHIGHI B., FALAHATI F.. INVOLVEMENT OF ESSENTIAL LYSINE RESIDUES IN THE CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYROGENASE PURIFIED FROM STREPTOMYCES AUREOF ACIENS. IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE[Internet]. 2007;31(A3):313-320. Available from: https://sid.ir/paper/117910/en

IEEE: Copy

B. HAGHIGHI, and F. FALAHATI, “INVOLVEMENT OF ESSENTIAL LYSINE RESIDUES IN THE CATALYTIC ACTIVITY OF GLUCOSE 6-PHOSPHATE DEHYROGENASE PURIFIED FROM STREPTOMYCES AUREOF ACIENS,” IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A- SCIENCE, vol. 31, no. A3, pp. 313–320, 2007, [Online]. Available: https://sid.ir/paper/117910/en

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