CHARACTERIZATION OF THE FULL LENGTH COAT PROTEIN GENE OF IRANIAN GRAPEVINE FANLEAF VIRUS ISOLATES, GENETIC VARIATION AND PHYLOGENETIC ANALYSIS

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SOKHANDAN BASHIR NEMAT; PASHAEI ALIREZA; DOULATI BANEH HAMED

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Journal: Iranian Journal of Biotechnology Year:2011 | Volume:9 | Issue:3 Page(s): 213-221

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Title

CHARACTERIZATION OF THE FULL LENGTH COAT PROTEIN GENE OF IRANIAN GRAPEVINE FANLEAF VIRUS ISOLATES, GENETIC VARIATION AND PHYLOGENETIC ANALYSIS

Author(s)

SOKHANDAN BASHIR NEMAT | PASHAEI ALIREZA | DOULATI BANEH HAMED | Issue Writer Certificate

Keywords

CLONINGQ2

COAT PROTEINQ3

DAS-ELISAQ2

GFLVQ2

PARSIMONIOUSQ2

RT-PCRQ2

SEQUENCINGQ2

Abstract

The full-length COAT PROTEIN gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized by reverse transcription polymerase chain reaction (RTPCR) and SEQUENCING. The expected 1515 bp COAT PROTEIN (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibody sandwich enzyme-linked immune sorbent assay (DASELISA) in a population of 330 symptomatic grapevine leaf samples. CP products of eight isolates were cloned and nucleotide sequences were determined.Parsimonious trees indicated that GFLV isolates from Iran formed a distinct cluster, suggesting an independent evolution.

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APA: Copy

SOKHANDAN BASHIR, NEMAT, PASHAEI, ALIREZA, & DOULATI BANEH, HAMED. (2011). CHARACTERIZATION OF THE FULL LENGTH COAT PROTEIN GENE OF IRANIAN GRAPEVINE FANLEAF VIRUS ISOLATES, GENETIC VARIATION AND PHYLOGENETIC ANALYSIS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 9(3), 213-221. SID. https://sid.ir/paper/139206/en

Vancouver: Copy

SOKHANDAN BASHIR NEMAT, PASHAEI ALIREZA, DOULATI BANEH HAMED. CHARACTERIZATION OF THE FULL LENGTH COAT PROTEIN GENE OF IRANIAN GRAPEVINE FANLEAF VIRUS ISOLATES, GENETIC VARIATION AND PHYLOGENETIC ANALYSIS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2011;9(3):213-221. Available from: https://sid.ir/paper/139206/en

IEEE: Copy

NEMAT SOKHANDAN BASHIR, ALIREZA PASHAEI, and HAMED DOULATI BANEH, “CHARACTERIZATION OF THE FULL LENGTH COAT PROTEIN GENE OF IRANIAN GRAPEVINE FANLEAF VIRUS ISOLATES, GENETIC VARIATION AND PHYLOGENETIC ANALYSIS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 3, pp. 213–221, 2011, [Online]. Available: https://sid.ir/paper/139206/en

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