SUITABILITY OF MRS-BILE AGAR FOR THE SELECTIVE ENUMERATION OF MIXED PROBIOTIC BACTERIA IN PRESENCE OF MESOPHILIC LACTIC ACID CULTURES AND YOGHURT BACTERIA

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SOHRABVANDI SARAH; MORTAZAVIAN AMIR MOHAMMAD; DOLATKHAHNEJAD MOHAMMAD REZA; BAHADORI MONFARED AYAD

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Journal: Iranian Journal of Biotechnology Year:2012 | Volume:10 | Issue:1 Page(s): 16-21

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Title

SUITABILITY OF MRS-BILE AGAR FOR THE SELECTIVE ENUMERATION OF MIXED PROBIOTIC BACTERIA IN PRESENCE OF MESOPHILIC LACTIC ACID CULTURES AND YOGHURT BACTERIA

Author(s)

SOHRABVANDI SARAH | MORTAZAVIAN AMIR MOHAMMAD | DOLATKHAHNEJAD MOHAMMAD REZA | BAHADORI MONFARED AYAD | Issue Writer Certificate

Keywords

ENUMERATIONQ2

MESOPHILICQ2

MRS-BILE AGARQ2

PROBIOTICQ2

Abstract

Measuring the viability of PROBIOTIC microorganisms in food products using plate count methodology is a common practice due to the simplicity (ease of performance), inexpensive and routine testing characters of this method. In present study, the suitability of de man rogosa and sharpe agar (MRS) bile agar medium for the selective ENUMERATION of mixed PROBIOTIC bacteria (Lactobacillus acidophilus LA-5, L. casei 431 and Bifidobacterium lactisBB-12) in presence of MESOPHILIC lactic cultures (Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris) and yoghurt bacteria (Streptococcus thermophilus and Lactobacillus delbrueckiissp. bulgaricus) was investigated. Yoghurt bacteria did not grow neither in presence of 0.15% nor 0.30% of bile salts, as was expected. MESOPHILIC lactic starters could grow at both concentrations of bile salts at all incubation temperatures except 37oC. According to these results, MRS-BILE AGAR (0.15 bile salts) could be successfully used for selective ENUMERATION of mixed PROBIOTIC cultures in presence of MESOPHILIC culture and/or yoghurt bacteria when plates were incubated at 37oC for 72 h.

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APA: Copy

SOHRABVANDI, SARAH, MORTAZAVIAN, AMIR MOHAMMAD, DOLATKHAHNEJAD, MOHAMMAD REZA, & BAHADORI MONFARED, AYAD. (2012). SUITABILITY OF MRS-BILE AGAR FOR THE SELECTIVE ENUMERATION OF MIXED PROBIOTIC BACTERIA IN PRESENCE OF MESOPHILIC LACTIC ACID CULTURES AND YOGHURT BACTERIA. IRANIAN JOURNAL OF BIOTECHNOLOGY, 10(1), 16-21. SID. https://sid.ir/paper/139252/en

Vancouver: Copy

SOHRABVANDI SARAH, MORTAZAVIAN AMIR MOHAMMAD, DOLATKHAHNEJAD MOHAMMAD REZA, BAHADORI MONFARED AYAD. SUITABILITY OF MRS-BILE AGAR FOR THE SELECTIVE ENUMERATION OF MIXED PROBIOTIC BACTERIA IN PRESENCE OF MESOPHILIC LACTIC ACID CULTURES AND YOGHURT BACTERIA. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2012;10(1):16-21. Available from: https://sid.ir/paper/139252/en

IEEE: Copy

SARAH SOHRABVANDI, AMIR MOHAMMAD MORTAZAVIAN, MOHAMMAD REZA DOLATKHAHNEJAD, and AYAD BAHADORI MONFARED, “SUITABILITY OF MRS-BILE AGAR FOR THE SELECTIVE ENUMERATION OF MIXED PROBIOTIC BACTERIA IN PRESENCE OF MESOPHILIC LACTIC ACID CULTURES AND YOGHURT BACTERIA,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 1, pp. 16–21, 2012, [Online]. Available: https://sid.ir/paper/139252/en

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