SHORT COMMUNICATION: A SIMPLE AND RAPID LEAF GENOMIC DNA EXTRACTION METHOD FOR POLYMERASE CHAIN REACTION ANALYSIS

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AMANI JAFAR; KAZEMI R.A.; ABBASI A.R.; SALMANIAN A.H.

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Journal: Iranian Journal of Biotechnology Year:2011 | Volume:9 | Issue:1 Page(s): 69-71

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Title

SHORT COMMUNICATION: A SIMPLE AND RAPID LEAF GENOMIC DNA EXTRACTION METHOD FOR POLYMERASE CHAIN REACTION ANALYSIS

Author(s)

AMANI JAFAR | KAZEMI R.A. | ABBASI A.R. | SALMANIAN A.H. | Issue Writer Certificate

Keywords

DNA EXTRACTIONQ2

LEAF TISSUEQ3

POLYSACCHARIDE REMOVALQ3

Abstract

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA EXTRACTION was optimized. This method use small amount of plant material to reduce inhibitory agents (alkaloids, phenolic). The procedure involves homogenization of the plant leaf in extraction buffer, incubation at 60°C, extraction by chloroform: iso-amyl alcohol and finally DNA precipitation by cold isopropanol. The results showed that the extracted DNA could be used directly for PCR.

Cites

DNA EXTRACTION OF ALMOND WITHOUT PHENOL AND LIQUID NITROGEN

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APA: Copy

AMANI, JAFAR, KAZEMI, R.A., ABBASI, A.R., & SALMANIAN, A.H.. (2011). SHORT COMMUNICATION: A SIMPLE AND RAPID LEAF GENOMIC DNA EXTRACTION METHOD FOR POLYMERASE CHAIN REACTION ANALYSIS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 9(1), 69-71. SID. https://sid.ir/paper/139279/en

Vancouver: Copy

AMANI JAFAR, KAZEMI R.A., ABBASI A.R., SALMANIAN A.H.. SHORT COMMUNICATION: A SIMPLE AND RAPID LEAF GENOMIC DNA EXTRACTION METHOD FOR POLYMERASE CHAIN REACTION ANALYSIS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2011;9(1):69-71. Available from: https://sid.ir/paper/139279/en

IEEE: Copy

JAFAR AMANI, R.A. KAZEMI, A.R. ABBASI, and A.H. SALMANIAN, “SHORT COMMUNICATION: A SIMPLE AND RAPID LEAF GENOMIC DNA EXTRACTION METHOD FOR POLYMERASE CHAIN REACTION ANALYSIS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 1, pp. 69–71, 2011, [Online]. Available: https://sid.ir/paper/139279/en

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