CLONING AND EXPRESSION OF VP2 GENE OF INFECTIOUS BURSAL DISEASE VIRUS IN EUKARYOTIC CELLS

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HOUSHYARIAN ROUZBEH; ROAYAEI ARDEKANI M.; GALEHDARI HAMID; SEYFIABAD SHAPOURI M.R.

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Journal: Iranian Journal of Biotechnology Year:2007 | Volume:5 | Issue:4 Page(s): 246-250

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Title

CLONING AND EXPRESSION OF VP2 GENE OF INFECTIOUS BURSAL DISEASE VIRUS IN EUKARYOTIC CELLS

Author(s)

HOUSHYARIAN ROUZBEH | ROAYAEI ARDEKANI M. | GALEHDARI HAMID | SEYFIABAD SHAPOURI M.R. | Issue Writer Certificate

Keywords

INFECTIOUS BURSAL DISEASEQ3

IBDVQ3

VP2Q3

EUKARYOTIC EXPRESSIONQ3

PCDNA4Q3

COS-7 CELL LINEQ3

Abstract

INFECTIOUS BURSAL DISEASE (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of INFECTIOUS BURSAL DISEASE virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. This region of 1356 bp was inserted into a EUKARYOTIC EXPRESSION plasmid, PCDNA4, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Plasmid DNA was transfected into COS-7 CELL LINE and transient expression of VP2 from the constructed plasmid was characterized by dot blotting with a polyclonal antibody to IBDV.

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APA: Copy

HOUSHYARIAN, ROUZBEH, ROAYAEI ARDEKANI, M., GALEHDARI, HAMID, & SEYFIABAD SHAPOURI, M.R.. (2007). CLONING AND EXPRESSION OF VP2 GENE OF INFECTIOUS BURSAL DISEASE VIRUS IN EUKARYOTIC CELLS. IRANIAN JOURNAL OF BIOTECHNOLOGY, 5(4), 246-250. SID. https://sid.ir/paper/139316/en

Vancouver: Copy

HOUSHYARIAN ROUZBEH, ROAYAEI ARDEKANI M., GALEHDARI HAMID, SEYFIABAD SHAPOURI M.R.. CLONING AND EXPRESSION OF VP2 GENE OF INFECTIOUS BURSAL DISEASE VIRUS IN EUKARYOTIC CELLS. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2007;5(4):246-250. Available from: https://sid.ir/paper/139316/en

IEEE: Copy

ROUZBEH HOUSHYARIAN, M. ROAYAEI ARDEKANI, HAMID GALEHDARI, and M.R. SEYFIABAD SHAPOURI, “CLONING AND EXPRESSION OF VP2 GENE OF INFECTIOUS BURSAL DISEASE VIRUS IN EUKARYOTIC CELLS,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 5, no. 4, pp. 246–250, 2007, [Online]. Available: https://sid.ir/paper/139316/en

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