مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Influence of ellagic acid on oxidative stress tolerance and rpoS gene expression in clinical isolates of Pseudomonas aeruginosa

Pages

  55-62

Abstract

 Due to the emergence of antibiotic-resistant clinical strains of Pseudomonas aeruginosa, there is an urgent need for a suitable and affordable alternative that is a non-antibiotic antimicrobial agent and creates a new generation of infectious disease treatment. In this regard, this study aims to investigate the antimicrobial potential of Ellagic acid against clinical strains of P. aeruginosa under physiological growth conditions and Oxidative stress. P. aeruginosa isolates from clinical samples were identified by biochemical tests and their antibiotic susceptibility was tested by disc diffusion method. The inhibitory effect of Ellagic acid against multiple drug resistance isolates was investigated by disc diffusion and broth microdilution methods and its effect on bacterial survival under physiological conditions and Oxidative stress was investigated by Time-Kill assay. The effect of Ellagic acid on rpoS gene expression was also investigated by the Real time-PCR method. In this study, Ellagic acid showed an inhibitory effect on the growth of all MDR isolates of P. aeruginosa tested. The minimum inhibitory concentration (MIC) of Ellagic acid in 5 isolates varied between 250 and 2000 µg/ml. Treatment with Ellagic acid rendered drug-resistant clinical strains of P. aeruginosa sensitive to Oxidative stress. It reduced the survival of P. aeruginosa, while treatment with 1/4MIC concentration of Ellagic acid resulted in a 4.7-fold decrease in log10 CFU/mL compared to the control. In the present study, treatment with a sub-inhibitory concentration of Ellagic acid also caused a significant decrease in rpoS gene expression (P˂0.05). The results of this study indicate that Ellagic acid inhibits the growth of P. aeruginosa and increases its sensitivity to Oxidative stress conditions. Conducting in vivo studies may clarify the possibility of its clinical application in the control of infections caused by resistant isolates of P. aeruginosa.

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