OPTIMIZATION OF ENVIRONMENTAL CONDITIONS FOR KEFIRAN PRODUCTION OF LACTOBACILLUS KEFIRANOFACIENS BY RESPONSE SURFACE METHODOLOGY

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ZANGANEH SAHAR; KHODAIYAN FARAMARZ; RAZAVI SEYED HADI; NAGHAVI MOHAMMAD REZA

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Journal: Iranian Journal of Biosystems Engineering Year:2017 | Volume:48 | Issue:3 Page(s): 329-336

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Title

OPTIMIZATION OF ENVIRONMENTAL CONDITIONS FOR KEFIRAN PRODUCTION OF LACTOBACILLUS KEFIRANOFACIENS BY RESPONSE SURFACE METHODOLOGY

Author(s)

ZANGANEH SAHAR | KHODAIYAN FARAMARZ | RAZAVI SEYED HADI | NAGHAVI MOHAMMAD REZA | Issue Writer Certificate

Keywords

EXOPOLY SACCHARIDEQ1

KEFIRANQ1

LACTOBACILLUS KEFIRANPFACIENSQ1

RESPONSE SURFACE METHODOLOGYQ2

Abstract

Production of Biopolymers by different microorganisms for industrial applications has been considered in recent years. Lactobacillus KEFIRANofaciens bacterium produces exopolysaccharid exists in kefir grains called KEFIRAN. The objective of this study was optimization of environmental conditions to produce KEFIRAN polysaccharide in a synthetic media based on whey substrate by using RESPONSE SURFACE METHODOLOGY (RSM). Therefore, different combinations of factors including pH (4.7-6.3), temperature (23-37oC) and agitation rate (70-170 rpm) on production of KEFIRAN (g/L) were studied. According to the results, the optimum condition for production of KEFIRAN included pH equal to 5.7, 31oC and agitation rate of 127 rpm which led to producing 1.14 g/L KEFIRAN after five days incubation period. Furthermore, the molecular weight of produced KEFIRAN in pure culture of L.kefiranofaciens measured by gel filtration chromatography was 1.1× 106 Da.

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APA: Copy

ZANGANEH, SAHAR, KHODAIYAN, FARAMARZ, RAZAVI, SEYED HADI, & NAGHAVI, MOHAMMAD REZA. (2017). OPTIMIZATION OF ENVIRONMENTAL CONDITIONS FOR KEFIRAN PRODUCTION OF LACTOBACILLUS KEFIRANOFACIENS BY RESPONSE SURFACE METHODOLOGY. IRANIAN JOURNAL OF BIOSYSTEMS ENGINEERING (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 48(3), 329-336. SID. https://sid.ir/paper/144298/en

Vancouver: Copy

ZANGANEH SAHAR, KHODAIYAN FARAMARZ, RAZAVI SEYED HADI, NAGHAVI MOHAMMAD REZA. OPTIMIZATION OF ENVIRONMENTAL CONDITIONS FOR KEFIRAN PRODUCTION OF LACTOBACILLUS KEFIRANOFACIENS BY RESPONSE SURFACE METHODOLOGY. IRANIAN JOURNAL OF BIOSYSTEMS ENGINEERING (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;48(3):329-336. Available from: https://sid.ir/paper/144298/en

IEEE: Copy

SAHAR ZANGANEH, FARAMARZ KHODAIYAN, SEYED HADI RAZAVI, and MOHAMMAD REZA NAGHAVI, “OPTIMIZATION OF ENVIRONMENTAL CONDITIONS FOR KEFIRAN PRODUCTION OF LACTOBACILLUS KEFIRANOFACIENS BY RESPONSE SURFACE METHODOLOGY,” IRANIAN JOURNAL OF BIOSYSTEMS ENGINEERING (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 48, no. 3, pp. 329–336, 2017, [Online]. Available: https://sid.ir/paper/144298/en

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