OPTIMIZATION OF PECTINLYASE PRODUCTION FROM DATE PULP BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY

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FARBEH F.; REZAZAD BARI M.; ALIZADEH KHALEDABAD M.

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Paper Information

Journal: JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ) Year:2011 | Volume:20/3 | Issue:2 Page(s): 13-22

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Title

OPTIMIZATION OF PECTINLYASE PRODUCTION FROM DATE PULP BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY

Author(s)

FARBEH F. | REZAZAD BARI M. | ALIZADEH KHALEDABAD M. | Issue Writer Certificate

Keywords

PECTINLYASEQ3

ASPERGILLUS NIGERQ2

OPTIMIZATIONQ3

DATE PULPQ2

Abstract

In this study a two-step approach was applied in order to investigate the effect of seven factors including: concentration of ammonium sulphate, potassium dihydrogen phosphate, DATE PULP, pH, inoculums size, agitation speed and fermentation time on the PECTINLYASE production by ASPERGILLUS NIGER. At first step, the effects of these variables were studied using fractional factorial design. According to obtained results three factors, pH, ammonium sulphate concentration and fermentation time were considered for further study. Second step was carried out by using central composite design (CCD) in order to find optimum conditions of pH, ammonium sulphate concentration and fermentation time. After analysis of CCD, optimum condition for maximization of PECTINLYASE activity determined as follow: ammonium sulphate concentration 0.35%, fermentation time, 73.93 hours and pH, 4.82. Enzyme activity at this optimum condition was 1.38 U. ml -1.

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APA: Copy

FARBEH, F., REZAZAD BARI, M., & ALIZADEH KHALEDABAD, M.. (2011). OPTIMIZATION OF PECTINLYASE PRODUCTION FROM DATE PULP BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), 20/3(2), 13-22. SID. https://sid.ir/paper/148611/en

Vancouver: Copy

FARBEH F., REZAZAD BARI M., ALIZADEH KHALEDABAD M.. OPTIMIZATION OF PECTINLYASE PRODUCTION FROM DATE PULP BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY. JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ)[Internet]. 2011;20/3(2):13-22. Available from: https://sid.ir/paper/148611/en

IEEE: Copy

F. FARBEH, M. REZAZAD BARI, and M. ALIZADEH KHALEDABAD, “OPTIMIZATION OF PECTINLYASE PRODUCTION FROM DATE PULP BY ASPERGILLUS NIGER USING RESPONSE SURFACE METHODOLOGY,” JOURNAL OF FOOD RESEARCH (UNIVERSITY OF TABRIZ), vol. 20/3, no. 2, pp. 13–22, 2011, [Online]. Available: https://sid.ir/paper/148611/en

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