MOLECULAR STUDY OF THECHLORELLA ALGAE AND DETERMINING ITS FUNCTIONAL FEATURES WITH THE APPROACH OF HYDROGENASE GENE EXPRESSION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOUSAVI SEYEDEH TAYEBEH; JAFARIAN VAHAB; BAHARI ABBAS

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Plant Biological Sciences Year:2016 | Volume:8 | Issue:29 Page(s): 21-36

Download Full-Text

Persian Verion

View:

557

Download:

Cites:

Information Journal Paper

Title

MOLECULAR STUDY OF THECHLORELLA ALGAE AND DETERMINING ITS FUNCTIONAL FEATURES WITH THE APPROACH OF HYDROGENASE GENE EXPRESSION

Author(s)

MOUSAVI SEYEDEH TAYEBEH | JAFARIAN VAHAB | BAHARI ABBAS | Issue Writer Certificate

Keywords

OPTIMIZATIONQ2

GENE EXPRESSIONQ2

CHLORELLA VULGARISQ1

REAL TIME PCRQ1

HYDROGENASEQ1

Abstract

Biohydrogen production by biological processes are known as a renewable energy source. The aim of the investigation was to find and optimize the most appropriate medium for the algae growth to produce the maximum amount of hydrogen. First of all, the bioinformatics and biosystematics studies were taken for identifying the collected microalgae which was detected as Chlorella with the following features: spherical appearance, spent protozoan, Cup-shaped chloroplast with no flagella. On the other hand, the molecular analysis by PCR and 18S sequence typing of interested microalgae demonstrated 100% similarity to that well known sequences for CHLORELLA VULGARIS. Second, we assessed some culture media including BBM, Chu10, TAP, and Sorokin and Krauss for optimum growth conditions for CHLORELLA VULGARIS. In general, our results showed that BBM medium had the highest efficiency for producing microalgae biomass under following conditions: pH=8, temperature of 30 ° with 16 to 8 h light to darkness periods ratio. Third, we designed a more efficient photobioreactor apparatus toward inducing more powerful bio-hydrogen production by HYDROGENASE enzyme activity of our given microalgae. Then, the performance of the apparatus as well as the GENE EXPRESSION was scrutinized under different conditions (light, pH, sulphorous, etc.). For this, after extracting RNA and constructing cDNA, HYDROGENASE gene was amplified with PCR and the product was evaluated by agarose gel. However, the relative expression of the gene measured by REAL TIME PCR showed the influence of light, pH and sulphourous on the expression as compared with control.

Cites

No record.

References

No record.

Cite

APA: Copy

MOUSAVI, SEYEDEH TAYEBEH, JAFARIAN, VAHAB, & BAHARI, ABBAS. (2016). MOLECULAR STUDY OF THECHLORELLA ALGAE AND DETERMINING ITS FUNCTIONAL FEATURES WITH THE APPROACH OF HYDROGENASE GENE EXPRESSION. IRANIAN JOURNAL OF PLANT BIOLOGY, 8(29 ), 21-36. SID. https://sid.ir/paper/160080/en

Vancouver: Copy

MOUSAVI SEYEDEH TAYEBEH, JAFARIAN VAHAB, BAHARI ABBAS. MOLECULAR STUDY OF THECHLORELLA ALGAE AND DETERMINING ITS FUNCTIONAL FEATURES WITH THE APPROACH OF HYDROGENASE GENE EXPRESSION. IRANIAN JOURNAL OF PLANT BIOLOGY[Internet]. 2016;8(29 ):21-36. Available from: https://sid.ir/paper/160080/en

IEEE: Copy

SEYEDEH TAYEBEH MOUSAVI, VAHAB JAFARIAN, and ABBAS BAHARI, “MOLECULAR STUDY OF THECHLORELLA ALGAE AND DETERMINING ITS FUNCTIONAL FEATURES WITH THE APPROACH OF HYDROGENASE GENE EXPRESSION,” IRANIAN JOURNAL OF PLANT BIOLOGY, vol. 8, no. 29 , pp. 21–36, 2016, [Online]. Available: https://sid.ir/paper/160080/en

Related Journal Papers