CALLUS AND SOMATIC EMBRYO INDUCTION IN DATE PALM CV. KHAZRAVI USING LEAVES, IMMATURE FRUIT AND ENDOSPERM EXPLANTS

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FARHADI KOLAHKAJ FATEME; POUR MOHAMMADI PAYAM; Farkhari Mohammad; ALAMI SAIED KHALIL

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Journal: Journal of Plant Biological Sciences Year:2018 | Volume:10 | Issue:36 Page(s): 89-101

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Title

CALLUS AND SOMATIC EMBRYO INDUCTION IN DATE PALM CV. KHAZRAVI USING LEAVES, IMMATURE FRUIT AND ENDOSPERM EXPLANTS

Author(s)

FARHADI KOLAHKAJ FATEME | POUR MOHAMMADI PAYAM | Farkhari Mohammad | ALAMI SAIED KHALIL | Issue Writer Certificate

Keywords

DATE PALM; AUXIN; CYTOKININ; CALLOGENESIS; SOMATIC EMBRYOGENESISQ2

Abstract

The purpose of this study was to check the possibility of callus and somatic embryogenesis in date palm cv. Khazravi using different explants including leaves, green fruit, immature seed, endosperm and green fruit pericarp. In the culture of leaves in the medium contains different plant growth regulators including 2, 4-D, BAP and TDZ, highest callus induction was achieved in medium supplemented with, 5 mg.L-1 2, 4-D, 2 mg.L-1 BAP and 4 mg.L-1 TDZ, and in treatment with 5 mg.L-1 2, 4-D, 1 mg.L-1 BAP and 4 mg.L-1 TDZ. By using explant of immature fruit, found that when two growths regulative, TDZ and 2, 4-D in amount of 5 mg.L-1 are in the culture medium callugenesis and embryogenesis was achieved. The endosperm cultivation with pericarp and embryo in the same medium showed that the presence of two growth regulators 2, 4-D and TDZ have been useful for callus production. Also in endosperm with zygotic embryo culture, embryo development and the root and shoot were formed after three months. Endosperm culture, in the culture medium containing various auxins and pericarp culture showed no any reactions. In general, these results showed that in date palm cv. Khazravi, treating the explants of leave and immature fruits with appropriate concentrations of plant growth regulators auxin and cytokinin, can induce callus and embryo.

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APA: Copy

FARHADI KOLAHKAJ, FATEME, POUR MOHAMMADI, PAYAM, Farkhari, Mohammad, & ALAMI SAIED, KHALIL. (2018). CALLUS AND SOMATIC EMBRYO INDUCTION IN DATE PALM CV. KHAZRAVI USING LEAVES, IMMATURE FRUIT AND ENDOSPERM EXPLANTS. IRANIAN JOURNAL OF PLANT BIOLOGY, 10(36 ), 89-101. SID. https://sid.ir/paper/160091/en

Vancouver: Copy

FARHADI KOLAHKAJ FATEME, POUR MOHAMMADI PAYAM, Farkhari Mohammad, ALAMI SAIED KHALIL. CALLUS AND SOMATIC EMBRYO INDUCTION IN DATE PALM CV. KHAZRAVI USING LEAVES, IMMATURE FRUIT AND ENDOSPERM EXPLANTS. IRANIAN JOURNAL OF PLANT BIOLOGY[Internet]. 2018;10(36 ):89-101. Available from: https://sid.ir/paper/160091/en

IEEE: Copy

FATEME FARHADI KOLAHKAJ, PAYAM POUR MOHAMMADI, Mohammad Farkhari, and KHALIL ALAMI SAIED, “CALLUS AND SOMATIC EMBRYO INDUCTION IN DATE PALM CV. KHAZRAVI USING LEAVES, IMMATURE FRUIT AND ENDOSPERM EXPLANTS,” IRANIAN JOURNAL OF PLANT BIOLOGY, vol. 10, no. 36 , pp. 89–101, 2018, [Online]. Available: https://sid.ir/paper/160091/en

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