ISOLATION AND CLONING OF CDNA MANGANESE PEROXIDASE GENE (MNP) FROM WHITE BUTTON MUSHROOM (AGARICUS BISPORUS), INTRODUCTION TO GENETIC MANIPULATION

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HASAN JANPOUR J.; FARSI MOHAMMAD

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Journal: Journal of Plant Biological Sciences Year:2010 | Volume:2 | Issue:1 (SEQUENCE 3) Page(s): 13-24

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Title

ISOLATION AND CLONING OF CDNA MANGANESE PEROXIDASE GENE (MNP) FROM WHITE BUTTON MUSHROOM (AGARICUS BISPORUS), INTRODUCTION TO GENETIC MANIPULATION

Author(s)

HASAN JANPOUR J. | FARSI MOHAMMAD | Issue Writer Certificate

Keywords

REVERSE TRANSCRIPTASE ENZYMEQ3

AGRICULTURE WASTESQ2

PTZ57R/T VECTORQ3

MOLECULAR CLONINGQ1

Abstract

Mushrooms, in addition to their nutritional values, are currently considered as useful tools for biological of agricultural wastes to useful food products. In the button mushroom (Agaricus bisporus), there are several enzymes which catalyze lignin compounds of the compost during the mycelia growth and the fructification phases. Manganese Peroxidase (MnP), as one of the most important lignin-degrading enzymes, plays a key role in degradation of lignin compounds in the button mushroom. To achieve a high yield of MnP in the A. bisporus, the gene encoding MnP was isolated, characterized and cloned. The total RNA was extracted from the mycelium growing on the liquid compost extract medium, followed by construction of its cDNA by reverse transcriptase. The PCR products were then inserted into the pTZ57R/T cloning vector, and transferred into E. coli (the DH5α strain). Finally, the plasmid was extracted from the transgenic bacteria, followed by enzymatic digestion and nucleotide sequencing. The BLAST analysis revealed two different nucleotides (657 and 850) between the cloned fragment (generated in this research) and the mnp1 (available in the gene bank of NCBI). The difference in the nucleotide positions of 657 and 850 subsequently changed Isoleucine to Valine and Serin to Alanine, respectively.

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References

PREPARATION OF AFLP MEDIATED - MOLECULAR CERTIFICATE FOR 12 BRED STRAINS OF THE BUTTON MUSHROOM, AGARICUS BISPORUS

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APA: Copy

HASAN JANPOUR, J., & FARSI, MOHAMMAD. (2010). ISOLATION AND CLONING OF CDNA MANGANESE PEROXIDASE GENE (MNP) FROM WHITE BUTTON MUSHROOM (AGARICUS BISPORUS), INTRODUCTION TO GENETIC MANIPULATION. IRANIAN JOURNAL OF PLANT BIOLOGY, 2(1 (SEQUENCE 3)), 13-24. SID. https://sid.ir/paper/160099/en

Vancouver: Copy

HASAN JANPOUR J., FARSI MOHAMMAD. ISOLATION AND CLONING OF CDNA MANGANESE PEROXIDASE GENE (MNP) FROM WHITE BUTTON MUSHROOM (AGARICUS BISPORUS), INTRODUCTION TO GENETIC MANIPULATION. IRANIAN JOURNAL OF PLANT BIOLOGY[Internet]. 2010;2(1 (SEQUENCE 3)):13-24. Available from: https://sid.ir/paper/160099/en

IEEE: Copy

J. HASAN JANPOUR, and MOHAMMAD FARSI, “ISOLATION AND CLONING OF CDNA MANGANESE PEROXIDASE GENE (MNP) FROM WHITE BUTTON MUSHROOM (AGARICUS BISPORUS), INTRODUCTION TO GENETIC MANIPULATION,” IRANIAN JOURNAL OF PLANT BIOLOGY, vol. 2, no. 1 (SEQUENCE 3), pp. 13–24, 2010, [Online]. Available: https://sid.ir/paper/160099/en

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