CLONING OF BKTB AND PHAC GENES EFFECTIVE IN PRODUCTION OF BACTERIAL BIODEGRADABLE BIOPOLYMERS

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AMIRMOZAFARI N.; MOHSENPOUR M.

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Paper Information

Journal: JOURNAL OF BIOLOGY SCIENCE Year:2010 | Volume:4 | Issue:2 (SERIES NO. 13) Page(s): 1-14

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Title

CLONING OF BKTB AND PHAC GENES EFFECTIVE IN PRODUCTION OF BACTERIAL BIODEGRADABLE BIOPOLYMERS

Author(s)

AMIRMOZAFARI N. | MOHSENPOUR M. | Issue Writer Certificate

Keywords

BACTERIAL BIOPOLYMERSQ3

BKT BQ3

PHA CQ3

RALSTONIA EUTROPHAQ3

Abstract

BACTERIAL BIOPOLYMERS (Polyhydroxyalkanoates (PHAs)) are polyesters synthesised by some bacteria as an intracellular carbon and energy storage compound. These are accumulated in the cytoplasm of cells. Because of its properties as biodegradable plastics, are of commercial importance. The aim of this study was isolation and cloning ofbkt B and PHA C genes fromRalstonia eutropha strain H16. The gene phaC encodes PHA polymerase enzyme which polymerase R-hydroxy butyryl-CoA and R-hydroxy valeryl-CoA subunits. The gene bktBencodes b ketothiolase enzyme which have an important role in the production of Rhydroxy valeryl-CoA and bacterial flexible copolymers formation. In this studybktB gene was cloned in the pGEM-T vector and transformed into competent cells that was prepared fromE. coli JM107. Insertion of this fragment was confirmed by using PCR and enzyme digestion. Specific primers (withSpe I and Nsi I restriction enzyme sites) were designed for isolation ofphaC gene.Then its entire ORF with RBS was amplified by PCR using R. eutrophagenomic DNA as template. This fragment after purification from agarose gel was cloned into pGEM-bktB vector and transformed intoE. coli BL21. Because of positive physical properties of copolymers in industry; the preparation of this recombinant bacterium could efficiently be used towards production of bioplastics with enhanced properties.

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References

ISOLATION OF BETAKETOTHIOLASE GENE (BKTB) FROM RALSTONIA EUTROPHA PTCC1615 IN ORDER TO MODIFIY BIODEGRADABLE PLASTICS PROPERTIES

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APA: Copy

AMIRMOZAFARI, N., & MOHSENPOUR, M.. (2010). CLONING OF BKTB AND PHAC GENES EFFECTIVE IN PRODUCTION OF BACTERIAL BIODEGRADABLE BIOPOLYMERS. JOURNAL OF BIOLOGY SCIENCE, 4(2 (SERIES NO. 13)), 1-14. SID. https://sid.ir/paper/164118/en

Vancouver: Copy

AMIRMOZAFARI N., MOHSENPOUR M.. CLONING OF BKTB AND PHAC GENES EFFECTIVE IN PRODUCTION OF BACTERIAL BIODEGRADABLE BIOPOLYMERS. JOURNAL OF BIOLOGY SCIENCE[Internet]. 2010;4(2 (SERIES NO. 13)):1-14. Available from: https://sid.ir/paper/164118/en

IEEE: Copy

N. AMIRMOZAFARI, and M. MOHSENPOUR, “CLONING OF BKTB AND PHAC GENES EFFECTIVE IN PRODUCTION OF BACTERIAL BIODEGRADABLE BIOPOLYMERS,” JOURNAL OF BIOLOGY SCIENCE, vol. 4, no. 2 (SERIES NO. 13), pp. 1–14, 2010, [Online]. Available: https://sid.ir/paper/164118/en

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