PRODUCTION AND ACTIVITY OF PERIPLASMIC ALKALINE PHOSPHATASE IN NATIVE E.COLI STRAINS ISOLATED FROM WATERS OF RUDSAR AREA

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FARKHONDEZIBA R.; GHAEMI N.; ASSMAR M.

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Journal: JOURNAL OF BIOLOGY SCIENCE Year:2010 | Volume:4 | Issue:1 (SERIES NO. 12) Page(s): 67-77

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Title

PRODUCTION AND ACTIVITY OF PERIPLASMIC ALKALINE PHOSPHATASE IN NATIVE E.COLI STRAINS ISOLATED FROM WATERS OF RUDSAR AREA

Author(s)

FARKHONDEZIBA R. | GHAEMI N. | ASSMAR M. | Issue Writer Certificate

Keywords

E.COLIQ3

ALKALINE PHOSPHATASEQ2

BIOSYNTHESISQ3

Abstract

The purpose of present survey was study the effective factors (determining induced and limiting factors) in BIOSYNTHESIS and activity of ALKALINE PHOSPHATASE in the native E.COLI strains. In this survey, 50 different wells from the city of Rudsar were sampled and inspected for existence of E.COLI. Following screening test, one strain capable of enzyme production was selected as a suitable strain. The specific substrate (4-Nitrophenyl phosphate disodium salt hexahydrate) was used for measuring enzyme activity. In present study the highest enzyme production was observed in base medium after 64 hours of growth and in optimum medium after 40 hours of growth in the early stationary phase. The most effective factors were as follows: Glucose 0.5% of carbon sources, Bactopeptone 0.5% of nitrogen sources, NaCl 0.6% of various concentrations of NaCl, Inoculation 6% of inoculation rate, 6%, pH 7.5 and 37oC temperature. The inorganic phosphate was appeared to be as limiting factors. The aerobic condition was best condition to production of enzyme and maximum activity of enzymen was at 37oC and pH 8.5. The thermal shock had best results for releasing the enzyme from periplasmic space. The endurable period of the enzyme obtained by thermal shock was 7 days in 2-8oC fridge temperature. The maximum enzyme production rate in optimum medium and base medium were 32.97 and 7.62 (U/ml/min) respectively.

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APA: Copy

FARKHONDEZIBA, R., GHAEMI, N., & ASSMAR, M.. (2010). PRODUCTION AND ACTIVITY OF PERIPLASMIC ALKALINE PHOSPHATASE IN NATIVE E.COLI STRAINS ISOLATED FROM WATERS OF RUDSAR AREA. JOURNAL OF BIOLOGY SCIENCE, 4(1 (SERIES NO. 12)), 67-77. SID. https://sid.ir/paper/164138/en

Vancouver: Copy

FARKHONDEZIBA R., GHAEMI N., ASSMAR M.. PRODUCTION AND ACTIVITY OF PERIPLASMIC ALKALINE PHOSPHATASE IN NATIVE E.COLI STRAINS ISOLATED FROM WATERS OF RUDSAR AREA. JOURNAL OF BIOLOGY SCIENCE[Internet]. 2010;4(1 (SERIES NO. 12)):67-77. Available from: https://sid.ir/paper/164138/en

IEEE: Copy

R. FARKHONDEZIBA, N. GHAEMI, and M. ASSMAR, “PRODUCTION AND ACTIVITY OF PERIPLASMIC ALKALINE PHOSPHATASE IN NATIVE E.COLI STRAINS ISOLATED FROM WATERS OF RUDSAR AREA,” JOURNAL OF BIOLOGY SCIENCE, vol. 4, no. 1 (SERIES NO. 12), pp. 67–77, 2010, [Online]. Available: https://sid.ir/paper/164138/en

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