STUDYING THE GENETIC DIVERSITY OF ANZALI WETLAND PIKEPERCH (SANDER LUCIOPERCA) USING MICROSATELLITE MARKERS

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GHARIBKHANI MAHTAB; POURKAZEMI MOHAMMAD; REZVANI GILKOLAI SOHRAB; TATINA MOSTAFA

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Journal: JOURNAL OF WETLAND ECOBIOLOGY Year:2011 | Volume:2 | Issue:7 Page(s): 3-10

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Title

STUDYING THE GENETIC DIVERSITY OF ANZALI WETLAND PIKEPERCH (SANDER LUCIOPERCA) USING MICROSATELLITE MARKERS

Author(s)

GHARIBKHANI MAHTAB | POURKAZEMI MOHAMMAD | REZVANI GILKOLAI SOHRAB | TATINA MOSTAFA | Issue Writer Certificate

Keywords

GENETIC DIVERSITYQ3

PIKEPERCHQ4

ANZALI WETLANDQ3

MARKERQ4

MICROSATELLITEQ4

Abstract

The aim of this study was to analysis the GENETIC DIVERSITY of ANZALI WETLAND PIKEPERCH (Sander lucioperca) based on MICROSATELLITE MARKERs. For this purpose, 50 samples of adult PIKEPERCH from ANZALI WETLAND were collected. For each sample, 2 g dorsal fin tissues was collected and conserved in absolute alcohol for subsequent DNA extraction and amplification. Total genomic DNA was extracted from fin tissue and the quality and concentration of DNA were assessed using agarose gel electrophoresis and spectrophotometer. Polymerase Chain Reaction (PCR) was conducted using 15 pairs of MICROSATELLITE primers. PCR products were separated on 6% polyacrylamide gels and stained by silver nitrate. DNA bands were analysed using Biocapt and GenAlex software package. Out of 15 pairs of MICROSATELLITE, 6 polymorphic loci were produced. The average number of alleles found per locus was 2.5. The range of observed and expected hetrozygosities were 0.30-0.80 and 0.25-0.63 respectively which the lowest amount was found in locus YP110 and the highest was found in locus Pfla L9. The low average number of alleles and GENETIC DIVERSITY is due to the artificial propagation in this species. Deviations from Hardy-Weinberg equilibrium were found in some of alleles that can be referred to non-accidental sampling because of small population size and inbreeding.

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APA: Copy

GHARIBKHANI, MAHTAB, POURKAZEMI, MOHAMMAD, REZVANI GILKOLAI, SOHRAB, & TATINA, MOSTAFA. (2011). STUDYING THE GENETIC DIVERSITY OF ANZALI WETLAND PIKEPERCH (SANDER LUCIOPERCA) USING MICROSATELLITE MARKERS. JOURNAL OF WETLAND ECOBIOLOGY, 2(7), 3-10. SID. https://sid.ir/paper/174813/en

Vancouver: Copy

GHARIBKHANI MAHTAB, POURKAZEMI MOHAMMAD, REZVANI GILKOLAI SOHRAB, TATINA MOSTAFA. STUDYING THE GENETIC DIVERSITY OF ANZALI WETLAND PIKEPERCH (SANDER LUCIOPERCA) USING MICROSATELLITE MARKERS. JOURNAL OF WETLAND ECOBIOLOGY[Internet]. 2011;2(7):3-10. Available from: https://sid.ir/paper/174813/en

IEEE: Copy

MAHTAB GHARIBKHANI, MOHAMMAD POURKAZEMI, SOHRAB REZVANI GILKOLAI, and MOSTAFA TATINA, “STUDYING THE GENETIC DIVERSITY OF ANZALI WETLAND PIKEPERCH (SANDER LUCIOPERCA) USING MICROSATELLITE MARKERS,” JOURNAL OF WETLAND ECOBIOLOGY, vol. 2, no. 7, pp. 3–10, 2011, [Online]. Available: https://sid.ir/paper/174813/en

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