Comparison of biochemical and Molecular Methods in Isolation and identification of Halophilic bacteria from saline Lake Inche boroun, Almagol and Ajigol in Golestan Province

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khastar elham; Jamali Aylar; NIK NEZHAD FARHAD

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Journal: JOURNAL OF WETLAND ECOBIOLOGY Year:2018 | Volume:10 | Issue:35 Page(s): 27-38

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Title

Comparison of biochemical and Molecular Methods in Isolation and identification of Halophilic bacteria from saline Lake Inche boroun, Almagol and Ajigol in Golestan Province

Author(s)

khastar elham | Jamali Aylar | NIK NEZHAD FARHAD | Issue Writer Certificate

Keywords

InchehborounQ2

AlmagolQ2

and Ajigol lakesQ2

tuf geneQ2

Staphylococcus epidermidisQ2

Staphylococcus capitisQ2

Staphylococcus hominisQ2

Abstract

Saline lakes with a salinity level close to that of seawater are extreme environments with a broad spectrum of microbial agents. The present study aimed at Comparison of biochemical and Molecular Methods in Isolation and identification of Halophilic bacteria from saline Lake Inche boroun, Almagol and Ajigol in Golestan Province. 51 samples were obtained from different regions of the above-mentioned lakes (seventeen samples from each lake) with varying EC and pH within a 4 month period from February to May 2015. The isolates were cultured on Saline nutrient broth and selected for a better identification. They were determined in terms of colony and cell morphology. Colony morphologies were specified with emphasis on pigment production, colony size, and turbidity. The properties were evaluated by culture growth in optimum EC, pH, and salinity. Biochemical tests of catalase, gelatinase, amylase, oxidase, citrate, urease, indole, Voges-Proskauer, fermentation/oxidation (lactose, sucrose, dextrose) and also PCR (tuf gene) were performed and the capability of producing the above-mentioned four enzymes was determined. According to the biochemical tests and molecular method, 22 halophile and extreme halophile isolates were separated from saline waters of Inchehboroun, Almagol, and Ajigol lakes where Staphylococcus epidermidis and Staphylococcus capitis were the most abundant species, respectively. Furthermore, three strains were able to produce the four enzymes; two strains belong to Staphylococcus capitis and one belongs to Staphylococcus hominis. Due to harsh living condition, saline environments have limited biological diversity compared to the so-called temperate environments. However, saline environments possess a high level of biological diversity in terms of halophile and halotolerant microorganisms with variety of relative halophiles and halotolerants living in these environments.

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APA: Copy

khastar, elham, Jamali, Aylar, & NIK NEZHAD, FARHAD. (2018). Comparison of biochemical and Molecular Methods in Isolation and identification of Halophilic bacteria from saline Lake Inche boroun, Almagol and Ajigol in Golestan Province. JOURNAL OF WETLAND ECOBIOLOGY, 10(35 ), 27-38. SID. https://sid.ir/paper/174823/en

Vancouver: Copy

khastar elham, Jamali Aylar, NIK NEZHAD FARHAD. Comparison of biochemical and Molecular Methods in Isolation and identification of Halophilic bacteria from saline Lake Inche boroun, Almagol and Ajigol in Golestan Province. JOURNAL OF WETLAND ECOBIOLOGY[Internet]. 2018;10(35 ):27-38. Available from: https://sid.ir/paper/174823/en

IEEE: Copy

elham khastar, Aylar Jamali, and FARHAD NIK NEZHAD, “Comparison of biochemical and Molecular Methods in Isolation and identification of Halophilic bacteria from saline Lake Inche boroun, Almagol and Ajigol in Golestan Province,” JOURNAL OF WETLAND ECOBIOLOGY, vol. 10, no. 35 , pp. 27–38, 2018, [Online]. Available: https://sid.ir/paper/174823/en

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